dc.contributor.author | Coleman, David | |
dc.contributor.author | Kinnevey, Peter | |
dc.date.accessioned | 2022-07-29T08:42:49Z | |
dc.date.available | 2022-07-29T08:42:49Z | |
dc.date.issued | 2022 | |
dc.date.submitted | 2022 | en |
dc.identifier.citation | Kinnevey PM, Kearney A, Shore AC, Earls MR, Brennan GI, Poovelikunnel TT, Humphreys H, Coleman DC. Meticillin-susceptible Staphylococcus aureus transmission among healthcare workers, patients and the environment in a large acute hospital under non-outbreak conditions investigated using whole-genome sequencing. Journal of Hospital Infection, 2022 May 18;127:15-25 | en |
dc.identifier.other | Y | |
dc.description.abstract | Background: The role of meticillin-susceptible Staphylococcus aureus (MSSA) colonization of healthcare workers (HCWs), patients and the hospital environment in MSSA transmission events (TEs) is poorly understood.
Aims: The role of meticillin-resistant Staphylococcus aureus (MRSA) was investigated recently under non-outbreak conditions in a large hospital with a history of endemic MRSA over 2 years using whole-genome sequencing (WGS). Numerous potential MRSA TEs were identified. The present study investigated MSSA TEs from the same sources during the same 2-year hospital study.
Methods: HCW (N=326) and patient (N=388) volunteers on nine wards were tested for nasal and oral MSSA colonization over 2 years. Near-patient environment (N=1164), high-frequency touch sites (N=810) and air (N=445) samples were screened for MSSA. Representative MSSA and clinical isolates were sequenced and analysed by core genome multi-locus sequence typing. Closely related isolates (≤24 allelic differences) were segregated into related isolate groups (RIGs). Potential TEs involving MSSA in RIGs from HCWs, patients and patient infections were identified in combination with epidemiological data.
Findings: In total, 635 MSSA were recovered: clinical isolates (N=82), HCWs (N=170), patients (N=120), and environmental isolates (N=263). Twenty-four clonal complexes (CCs) were identified among 406/635 MSSA sequenced, of which 183/406 segregated into 59 RIGs. Numerous potential HCW-to-patient, HCW-to-HCW and patient-to-patient TEs were identified, predominantly among CC5-MSSA, CC30-MSSA and CC45-MSSA. HCW, patient, clinical and environmental isolates were identified in 33, 24, six and 32 RIGs, respectively, with 19/32 of these containing MSSA related to HCW and/or patient isolates.
Conclusions: WGS detected numerous potential hospital MSSA TEs involving HCWs, patients and environmental contamination under non-outbreak conditions. | en |
dc.format.extent | 15 | en |
dc.format.extent | 25 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | JOURNAL OF HOSPITAL INFECTION; | |
dc.relation.ispartofseries | 127; | |
dc.rights | Y | en |
dc.subject | Whole-genome sequencing | en |
dc.subject | Oral colonization | en |
dc.subject | Non-outbreak conditions | en |
dc.subject | Meticillin-susceptible Staphylococcus aureus | en |
dc.subject | Nasal colonization | en |
dc.subject | Environmental contamination | en |
dc.subject | Hospital transmission | en |
dc.subject | MSSA | en |
dc.subject | MSSA colonization | en |
dc.title | Meticillin-susceptible Staphylococcus aureus transmission among healthcare workers, patients and the environment in a large acute hospital under non-outbreak conditions investigated using whole-genome sequencing | en |
dc.title.alternative | Transmission of MSSA under non-outbreak conditions | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/dcoleman | |
dc.identifier.peoplefinderurl | http://people.tcd.ie/kinnevp | |
dc.identifier.rssinternalid | 243378 | |
dc.identifier.doi | https://doi.org/10.1016/j.jhin.2022.05.004 | |
dc.rights.ecaccessrights | openAccess | |
dc.subject.TCDTheme | Genes & Society | en |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.subject.TCDTag | Hospital transmission events | en |
dc.subject.TCDTag | Methicillin-susceptible Staphylococcus aureus | en |
dc.subject.TCDTag | Oral carriage of Staphylococcus aureus | en |
dc.subject.TCDTag | whole-genome sequencing | en |
dc.identifier.rssuri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=35594983&dopt=Abstract | |
dc.identifier.orcid_id | 0000-0003-1797-2888 | |
dc.subject.darat_thematic | Health | en |
dc.status.accessible | N | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |
dc.contributor.sponsorGrantNumber | HRA-POR-2015-1051 | en |
dc.identifier.uri | http://hdl.handle.net/2262/100344 | |