AN EMERGING PANTON-VALENTINE (PVL)-POSITIVE CC5-METICILLIN-RESISTANT Staphylococcus aureus-IVc CLONE RECEOVERED FROM HOSPITAL AND COMMUNITY SETTINGS OVER A 17-YEAR PERIOD FROM 12 COUNTRIES INVESTIGATED BY WHOLE-GENOME SEQUENCING
File Type:
PDFItem Type:
Journal ArticleDate:
2023Access:
openAccessCitation:
ALOBA BK, KINNEVEY PM, MONECKE S, BRENNAN GI, O'CONNELL B, BLOMFELDT A, McMANUS B, SCHNEIDER-BRACHERT W, TKADLEC J, EHRICHT R, SENOK A, DAMKJAER BARTELS M, COLEMAN DC, AN EMERGING PANTON-VALENTINE (PVL)-POSITIVE CC5-METICILLIN-RESISTANT Staphylococcus aureus-IVc CLONE RECEOVERED FROM HOSPITAL AND COMMUNITY SETTINGS OVER A 17-YEAR PERIOD FROM 12 COUNTRIES INVESTIGATED BY WHOLE-GENOME SEQUENCING, JOURNAL OF HOSPITAL INFECTION, 132, February, 2023, 8 - 19Download Item:
Abstract:
Background
A novel Panton–Valentine leukocidin (PVL)-positive meticillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC)5-MRSA-IVc (‘Sri Lankan’ clone) was recently described from Sri Lanka. Similar isolates caused a recent Irish hospital outbreak.
Aim
To investigate the international dissemination and diversity of PVL-positive CC5-MRSA-IVc isolates from hospital and community settings using whole-genome sequencing (WGS).
Methods
Core-genome single nucleotide polymorphism (cgSNP) analysis, core-genome multi-locus sequence typing (cgMLST) and microarray-based detection of antimicrobial-resistance and virulence genes were used to investigate PVL-positive CC5-MRSA-IVc (N = 214 including 46 ‘Sri Lankan’ clone) from hospital and community settings in 12 countries over 17 years. Comparators included 29 PVL-positive and 23 PVL-negative CC5/ST5-MRSA-I/II/IVa/IVc/IVg/V.
Results
Maximum-likelihood cgSNP analysis grouped 209/214 (97.7%) CC5-MRSA-IVc into Clade I; average of 110 cgSNPs between isolates. Clade III contained the five remaining CC5-MRSA-IVc; average of 92 cgSNPs between isolates. Clade II contained seven PVL-positive CC5-MRSA-IVa comparators, whereas the remaining 45 comparators formed an outlier group. Minimum-spanning cgMLST analysis revealed a comparably low average of 57 allelic differences between all CC5/ST5-MRSA-IVc. All 214 CC5/ST5-MRSA-IVc were identified as ‘Sri Lankan’ clone, predominantly spa type t002 (186/214) with low population diversity and harboured a similar range of virulence genes and variable antimicrobial-resistance genes. All 214 Sri Lankan clone isolates and Clade II comparators harboured a 9616-bp chromosomal PVL-encoding phage remnant, suggesting both arose from a PVL-positive meticillin-susceptible ancestor. Over half of Sri Lankan clone isolates were from infections (142/214), and where detailed metadata were available (168/214), most were community associated (85/168).
Conclusions
Stable chromosomal retention of pvl may facilitate Sri-Lankan clone dissemination.
Author's Homepage:
http://people.tcd.ie/dcolemanhttp://people.tcd.ie/kinnevp
Description:
PUBLISHED5th December 2022; Pub ahead of print
Author: Coleman, David; Kinnevey, Peter
Type of material:
Journal ArticleCollections
Series/Report no:
JOURNAL OF HOSPITAL INFECTION132
February
Availability:
Full text availableKeywords:
CC5-MRSA-IVc, Dissemination, Epidemiology, Phylogenomics, PVL, Sri Lankan cloneSubject (TCD):
Genes & Society , Immunology, Inflammation & Infection , Long Read Sequencing , MRSA , STAPHYLOCOCCUS AUREUS INFECTION , whole-genome sequencingDOI:
https://doi.org/10.1016/j.jhin.2022.11.015Metadata
Show full item recordLicences: