Effect of Extraction Methods on Essential Oil Composition: A Case Study of Irish Bog Myrtle -Myrica gale L.

Abstract

Myrica gale is an aromatic peatland shrub that has reported traditional use as an insect repellent. Different extraction methodologies were used in this study to isolate the essential oil of Myrica gale L., including Clevenger hydrodistillation (CH) and microwave-assisted hydrodistillation (MAH). The oils, isolated from different plant parts (leaves, fruit and branches) collected in summer and autumn, were analysed by GC-MS and the volatiles from plant tissue were directly analysed by headspace-GC-MS. A total of 58 components were identified, including 15 monoterpene hydrocarbons (22.78–98.98%), 14 oxygenated monoterpenes (0.91–43.02%), 13 sesquiterpene hydrocarbons (0.05–24.98%), 3 oxygenated sesquiterpenes (0.07–13.16%) and 13 other compounds (0.05–5.21%). Headspace sampling furnished monoterpenes, while CH and MAH extracted monoterpenes and sesquiterpenes, with α-pinene (6.04–70.45%), eucalyptol (0.61–33.80%), limonene (2.27–20.73%) and α-phellandrene (2.33–15.61%) as major components in all plant parts. Quantitative differences occurred between extraction methodologies, with MAH yielding higher quantities of monoterpene and sesquiterpene hydrocarbons and CH targeting oxygenated counterparts. Leaves gave more complex chemical fingerprints than branches and fruit, and the summer collection yielded more components than the autumn collections. An OPLS-DA model was applied to the GC-MS data to compare the chemical profiles based on the extraction techniques and plant parts, and molecular networks were obtained for monoterpenes and sesquiterpenes connected via biosynthetic pathways. The essential oil profile of Myrica gale was influenced by the season of collection, plant part and extraction method.