dc.contributor.author | Sullivan, Derek | en |
dc.contributor.author | Moran, Gary | en |
dc.date.accessioned | 2010-10-14T13:54:55Z | |
dc.date.available | 2010-10-14T13:54:55Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | O'Connor, L., Caplice, N., Coleman, D. C., Sullivan, D.J. and Moran, G.P., Differential filamentation of Candida albicans and C. dubliniensis is governed by nutrient regulation of UME6 expression, Eukaryotic Cell, 9, 2010, 1383 - 1397 | en |
dc.identifier.other | Y | en |
dc.description | PUBLISHED | en |
dc.description.abstract | Candida dubliniensis is closely related to C. albicans, however it is responsible for
fewer infections in humans and is less virulent in animal models of infection. C.
dubliniensis forms fewer hyphae in vivo and this may contribute to its reduced
virulence. In this study we show that unlike C. albicans, C. dubliniensis fails to form
hyphae in YPD supplemented with 10% (v/v) fetal calf serum (YPDS). However, C.
dubliniensis filaments in water plus 10% (v/v) fetal calf serum (WS), and this
filamentation is inhibited by the addition of peptone and glucose. Repression of
filamentation in YPDS could be partly overcome by preculture in synthetic Lee?s
medium. Unlike C. albicans, inoculation of C. dubliniensis in YPDS did not result in
increased UME6 transcription. However, >100-fold induction of UME6 was observed
when C. dubliniensis was inoculated in nutrient poor WS medium. Addition of
increasing concentrations of peptone to WS had a dose dependent effect on reducing
UME6 expression. Transcript profiling of C. dubliniensis hyphae in WS identified a
starvation resposne involving expression of genes in the glyoxylate cycle and fatty
acid oxidation. In addition a core, shared transcriptional response with C. albicans
could be identified, including expression of virulence-associated genes including
SAP456, SAP7, HWP1 and SOD5. Preculture in nutrient limiting medium enhanced
adherence of C. dubliniensis, epithelial invasion and survival following co-culture
with murine macrophages. In conclusion, C. albicans unlike C. dubliniensis, appears
to form hyphae in liquid medium regardless of nutrient availability, which may
account for its increased capacity to cause disease in humans. | en |
dc.description.sponsorship | Irish Health Research Board (Research Grant RP/2004/235) and Science
Foundation Ireland (Programme Investigator grant no. 04/IN3/B463). | en |
dc.format.extent | 1383 | en |
dc.format.extent | 1397 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | Eukaryotic Cell | en |
dc.relation.ispartofseries | 9 | en |
dc.rights | Y | en |
dc.subject | Candida albicans | en |
dc.subject | Candida dubliniensis | en |
dc.subject | regulation | en |
dc.subject | UME6 | en |
dc.title | Differential filamentation of Candida albicans and C. dubliniensis is governed by nutrient regulation of UME6 expression | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/djsullvn | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/gmoran | en |
dc.identifier.rssinternalid | 67805 | en |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.identifier.rssuri | http://dx.doi.org/10.1128/EC.00042-10 | en |
dc.identifier.orcid_id | 0000-0003-0195-9697 | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |
dc.identifier.uri | http://hdl.handle.net/2262/41042 | |