dc.contributor.author | COLEMAN, DAVID | en |
dc.contributor.author | BRENNAN, ORLA | en |
dc.contributor.author | SHORE, ANNA | en |
dc.date.accessioned | 2010-11-24T12:22:26Z | |
dc.date.available | 2010-11-24T12:22:26Z | |
dc.date.issued | 2010 | en |
dc.date.submitted | 2010 | en |
dc.identifier.citation | SHORE AC, BRENNAN OM, EHRICHT R, MONECKE S, SCHWARZ S, SLICKERS P, COLEMAN DC, IDENTIFICATION AND CHARACTERIZATION OF THE MULTIDRUG RESISTANCE GENE cfr IN PANTON-VALENTINE LEUKOCIDIN-POSITIVE ST8-METHICILLIN-RESISTANT Staphylococcus aureus-IVa (USA300), ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 54, 12, 2010, 4978 - 4984 | en |
dc.identifier.other | Y | en |
dc.description | PUBLISHED | en |
dc.description | 4 October; Epub ahead of print | en |
dc.description.abstract | The staphylococcal cfr gene mediates resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A, a phenotype that has been termed PhLOPSA. The cfr gene has mainly been associated with coagulase-negative staphylococcal isolates from animals, and only a few cfr-positive methicillin-resistant Staphylococcus aureus (MRSA) isolates have been described so far. This study reports the first description of a cfr-positive MRSA isolate (M05/0060) belonging to the pandemic Panton-Valentine leukocidin (PVL)-positive sequence type 8 MRSA IVa/USA300 (ST8-MRSA-IVa/USA300) clone. The cfr gene was detected in M05/0060 using a DNA microarray which was used to screen PVL-positive MRSA isolates for the presence of virulence genes, typing markers, and antimicrobial resistance genes. Antimicrobial susceptibility testing revealed that M05/0060 exhibited the cfr-associated resistance phenotype. Molecular analysis identified the presence of cfr and a second phenicol resistance gene, fexA, on a novel 45-kb conjugative plasmid, which was designated pSCFS7. Within pSCFS7, a DNA segment consisting of cfr, a truncated copy of insertion sequence IS21-558, and a region with homology to the DNA invertase gene bin3 of transposon Tn552 from Bacillus mycoides was integrated into the transposase gene tnpB of the fexA-carrying transposon Tn558. The emergence of a multidrug-resistant cfr-positive variant of ST8-MRSA-IVa/USA300 is alarming and requires ongoing surveillance. Moreover, the identification of a novel conjugative plasmid carrying the cfr gene indicates the ability of cfr to spread to other MRSA strains. | en |
dc.format.extent | 4978 | en |
dc.format.extent | 4984 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | en |
dc.relation.ispartofseries | 54 | en |
dc.relation.ispartofseries | 12 | en |
dc.rights | Y | en |
dc.subject | Immunology | en |
dc.subject | Staphylococcus aureus | en |
dc.title | IDENTIFICATION AND CHARACTERIZATION OF THE MULTIDRUG RESISTANCE GENE cfr IN PANTON-VALENTINE LEUKOCIDIN-POSITIVE ST8-METHICILLIN-RESISTANT Staphylococcus aureus-IVa (USA300) | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/dcoleman | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/ashore | en |
dc.identifier.rssinternalid | 68824 | en |
dc.identifier.doi | http://dx.doi.org/10.1128/AAC.01113-10 | en |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.identifier.rssuri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20921317&dopt=Abstract | en |
dc.identifier.orcid_id | 0000-0003-1797-2888 | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |
dc.contributor.sponsorGrantNumber | TRA/2006/4 | en |
dc.identifier.uri | http://hdl.handle.net/2262/41227 | |