dc.contributor.author | SENGE, MATHIAS | en |
dc.contributor.author | EHRHARDT, CARSTEN | en |
dc.contributor.author | PASZKO, EDYTA | en |
dc.date.accessioned | 2013-10-21T13:58:37Z | |
dc.date.available | 2013-10-21T13:58:37Z | |
dc.date.issued | 2013 | en |
dc.date.submitted | 2013 | en |
dc.identifier.citation | E Paszko, GM Vaz, C Ehrhardt, MO Senge, Transferrin conjugation does not increase the efficiency of liposomal Foscan during in vitro photodynamic therapy of oesophageal cancer, European Journal of Pharmaceutical Sciences, 48, 1-2, 2013, 202-210 | en |
dc.identifier.other | Y | en |
dc.description | PUBLISHED | en |
dc.description.abstract | Photodynamic therapy (PDT) is based on the delivery of photocytotoxic agents to a target
tissue, followed by irradiation. In order to increase the efficiency of PDT in oesophageal
cancer therapy, polyethylene glycol (PEG)-grafted, transferrin (Tf)-conjugated liposome
formulations of 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (Foscan), a second-generation
photosensitiser, were prepared. Expression of transferrin receptors (CD71) in the oesophageal
cancer cell line, OE21, was confirmed by immunoblot and confocal laser scanning
microscopy. The anti-proliferative effect of Foscan liposomes was evaluated and compared
with plain formulations (i.e., without Tf) as well as with free drug. In addition, the
intracellular accumulation was studied using high content analysis. Surprisingly, delivering
Foscan by transferrin-conjugated PEG-liposomes to oesophageal cancer cells did not improve
the photocytotoxicity or the intracellular accumulation of Foscan when compared to
unmodified liposomes or indeed free photosensitiser. Tf-targeted drugs and drug delivery
systems have shown improvedthe therapy of many cancers. Our study, however, did not
corroborate these findings. If this is due to the tumour type, the choice of in vitro model or
the delivery systems remains to be confirmed. | en |
dc.description.sponsorship | This work was supported by grants from the Health Research Board (translational research
award 2007 TRA/2007/11), Science Foundation Ireland (SFI P.I. 09/IN.1/B2650 and SRC
(07/SRC/B1154) and Fundac?o da Ciencia e Tecnologia (SFRH/BD/62364/2009). The
authors wish to thank to Elena Schwagerus for assistance in the Western blot and Jennifer
Conroy for support with confocal laser scanning microscopy | en |
dc.format.extent | 202-210 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | European Journal of Pharmaceutical Sciences | en |
dc.relation.ispartofseries | 48 | en |
dc.relation.ispartofseries | 1-2 | en |
dc.rights | Y | en |
dc.subject.other | Drug targeting | |
dc.subject.other | Drug targeting | |
dc.title | Transferrin conjugation does not increase the efficiency of liposomal Foscan during in vitro photodynamic therapy of oesophageal cancer | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/ehrhardc | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/sengem | en |
dc.identifier.rssinternalid | 81162 | en |
dc.identifier.doi | http://dx.doi.org/10.1016/j.ejps.2012.10.018 | en |
dc.rights.ecaccessrights | OpenAccess | |
dc.subject.TCDTheme | Cancer | en |
dc.identifier.orcid_id | 0000-0003-0730-1829 | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |
dc.identifier.uri | http://hdl.handle.net/2262/67518 | |