Browsing RSS Feeds by Author "CAFFREY, MARTIN"
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Automatic Mixing of Bio-Samples Using Micro-Channel and Centrifugation
CAFFREY, MARTIN (IEEE, 2007)Automatic mixing of bio-samples using micro-channel and centrifugation is considered. Existing methods for mixing bio-samples for life science applications use micro-well and micro-stirrer which are ineffective when used ... -
Bernal and the genesis of structural biology.
CAFFREY, MARTIN (2007)I was invited to participate in this Symposium a month or so before the event. At that time however, I knew little about J D Bernal. I vaguely remembered a brief conversation on the topic over a decade ago with Professor ... -
Calorimetric and x-ray diffraction studies of rye glucocerebroside mesomorphism
CAFFREY, MARTIN (1992)Glucocerebrosides (GlcCer) isolated from the leaves of winter rye (Secale cereale L. cv Puma) differ from the more commonly investigated natural and synthetic cerebrosides, in that greater than 95% of the fatty acids are ... -
Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination
CAFFREY, MARTIN; BOLAND, COILIN (2014)Membrane proteins are key elements in cell physiology and drug targeting, but getting a high-resolution structure by crystallographic means is still enormously challenging. Novel strategies are in big demand to facilitate ... -
Cloning, expression, purification, crystallization and preliminary X-ray diffraction of a lysine-specific permease from Pseudomonas aeruginosa
CAFFREY, MARTIN (2014)The prokaryotic lysine-specific permease (LysP) belongs to the amino acid– polyamine–organocation (APC) transporter superfamily. In the cell, members of this family are responsible for the uptake and recycling of nutrients, ... -
A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes
CAFFREY, MARTIN (2015)The lipid cubic phase or in meso method is a robust approach for crystallizing membrane proteins for structure determination. The uptake of the method is such that it is experiencing what can only be described ... -
A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa
CAFFREY, MARTIN (2014)The exopolysaccharide alginate is an important component of biofilms produced by Pseudomonas aeruginosa , a major pathogen that contributes to the demise of cystic fibrosis patients. Alginate exits the cell via the ... -
Crystal structure of the human 5-HT<inf>1B</inf>serotonin receptor bound to an inverse agonist
CAFFREY, MARTIN (2018) -
Crystal structure of the integral membrane diacylglycerol kinase
CAFFREY, MARTIN (2013)Diacylglycerol kinase catalyses the ATP-dependent phosphorylation of diacylglycerol to phosphatidic acid for use in shuttling water-soluble components to membrane-derived oligosaccharide and lipopolysaccharide in the cell ... -
Crystallization and preliminary X-ray diffraction analysis of a soluble domain of the putative zinc transporter CzrB from Thermus thermophilus
CAFFREY, MARTIN (2007)CzrB is a putative zinc transporter from Thermus thermophilus. The protein is proposed to consist of a hexahelical transmembrane domain with a cytosolic extramembranal C-terminus. The latter 92-residue fragment may be ... -
Crystallizing membrane proteins for structure determination using lipidic mesophases.
CAFFREY, MARTIN (2010)A detailed protocol for crystallizing membrane proteins by using lipidic mesophases is described. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be ... -
Crystallizing membrane proteins for structure-function studies using lipidic mesophases.
CAFFREY, MARTIN (2011)The lipidic cubic phase method for crystallizing membrane proteins has posted some high-profile successes recently. This is especially true in the area of G-protein-coupled receptors, with six new crystallographic ... -
Crystallizing Membrane Proteins in Lipidic Mesophases. A Host Lipid Screen
CAFFREY, MARTIN (2011)The default lipid for the bulk of the crystallogenesis studies performed to date using the cubic mesophase method is monoolein. There is no good reason however, why this 18-carbon, cis - monounsaturated monoacylglycerol ... -
Crystallizing Membrane Proteins in the Lipidic Mesophase. Experience with Human Prostaglandin E2 Synthase 1 and an Evolving Strategy
CAFFREY, MARTIN (2014)The lipidic mesophase or in meso method for crystallizing membrane proteins has several high profile targets to its credit and is growing in popularity. Despite its success, the method is in its infancy as far as rational ... -
Crystallizing membrane proteins using lipidic mesophases.
CAFFREY, MARTIN (2009)A detailed protocol for crystallizing membrane proteins that makes use of lipidic mesophases is described. This has variously been referred to as the lipid cubic phase or in meso method. The method has been shown to be ... -
Crystallizing Transmembrane Peptides in Lipidic Mesophases
CAFFREY, MARTIN (2010)Structure determination of membrane proteins by crystallographic means has been facilitated by crystallization in lipidic mesophases. It has been suggested, however, that this so-called in meso method, as originally ... -
Data-collection strategy for challenging native SAD phasing
CAFFREY, MARTIN (2016) -
Detergents Destabilize the Cubic Phase of Monoolein. Implications for Membrane Protein Crystallization.
CAFFREY, MARTIN (Elsevier, 2003)The in meso method for membrane protein crystallization uses a lipidic cubic phase as the hosting medium. The cubic phase provides a lipid bilayer into which the protein presumably reconstitutes and from which protein ... -
Direct correlation of structure changes and thermal events in hydrated lipid established by simultaneous calorimetry and time-resolved x-ray diffraction.
CAFFREY, MARTIN (1992)In many lipid systems, polymorphic and mesomorphic behavior depends on sample thermal history. To establish unequivocally the structural origin of endothermic and exothermic events in such systems, we have performed ...