Docetaxel-resistance in prostate cancer: evaluating associated phenotypic changes and potential for resistance transfer via exosomes.

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Journal ArticleDate:
2012Access:
openAccessCitation:
Corcoran C, Rani S, O'Brien K, O'Neill A, Prencipe M, Sheikh R, Webb G, Mc Dermott R, Watson W, Crown J, O'Driscoll L., Docetaxel-resistance in prostate cancer: evaluating associated phenotypic changes and potential for resistance transfer via exosomes., PloS One, 7, 12, 2012, e50999-51009Download Item:
Abstract:
Background
Hormone-refractory prostate cancer remains hindered by inevitable progression of resistance to first-line treatment with docetaxel. Recent studies suggest that phenotypic changes associated with cancer may be transferred from cell-to-cell via microvesicles/exosomes. Here we aimed to investigate phenotypic changes associated with docetaxel-resistance in order to help determine the complexity of this problem and to assess the relevance of secreted exosomes in prostate cancer.
Methodology/Principal Findings
Docetaxel-resistant variants of DU145 and 22Rv1 were established and characterised in terms of cross-resistance, morphology, proliferation, motility, invasion, anoikis, colony formation, exosomes secretion their and functional relevance. Preliminary analysis of exosomes from relevant serum specimens was also performed. Acquired docetaxel-resistance conferred cross-resistance to doxorubicin and induced alterations in motility, invasion, proliferation and anchorage-independent growth. Exosomes expelled from DU145 and 22Rv1 docetaxel-resistant variants (DU145RD and 22Rv1RD) conferred docetaxel-resistance to DU145, 22Rv1 and LNCap cells, which may be partly due to exosomal MDR-1/P-gp transfer. Exosomes from prostate cancer patients’ sera induced increased cell proliferation and invasion, compared to exosomes from age-matched controls. Furthermore, exosomes from sera of patients undergoing a course of docetaxel treatment compared to matched exosomes from the same patients prior to commencing docetaxel treatment, when applied to both DU145 and 22Rv1 cells, showed a correlation between cellular response to docetaxel and patients’ response to treatment with docetaxel.
Conclusions/Significance
Our studies indicate the complex and multifaceted nature of docetaxel-resistance in prostate cancer. Furthermore, our in vitro observations and preliminary clinical studies indicate that exosomes may play an important role in prostate cancer, in cell-cell communication, and thus may offer potential as vehicles containing predictive biomarkers and new therapeutic targets.
Sponsor
Grant Number
Science Foundation Ireland (SFI)
08/ SRC/ B1410
Author's Homepage:
http://people.tcd.ie/lodriscDescription:
PUBLISHEDdoi: 10.1371/journal.pone.0050999 PMID: 23251413
Author: CORCORAN, CLAIRE; O'DRISCOLL, LORRAINE
Sponsor:
Science Foundation Ireland (SFI)Type of material:
Journal ArticleSeries/Report no:
PloS One7
12
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Full text availableSubject:
Cancer treatmentSubject (TCD):
Cancer , Drug discovery, profiling, targetingDOI:
http://dx.doi.org/10.1371/journal.pone.0050Metadata
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