Show simple item record

dc.contributor.authorGEGENBAUER, KRISTINAen
dc.date.accessioned2014-12-01T13:47:01Z
dc.date.available2014-12-01T13:47:01Z
dc.date.issued2013en
dc.date.submitted2013en
dc.identifier.citationGegenbauer K, Nagy Z, Smolenski A, Cyclic nucleotide dependent dephosphorylation of regulator of G-protein signaling 18 in human platelets., PloS one, 8, 11, 2013, e80251en
dc.identifier.otherYen
dc.descriptionPUBLISHEDen
dc.description.abstractRegulator of G-protein signaling 18 (RGS18) is a GTPase-activating protein that turns off Gq signaling in platelets. RGS18 is regulated by binding to the adaptor protein 14-3-3 via phosphorylated serine residues S49 and S218 on RGS18. In this study we confirm that thrombin, thromboxane A2, or ADP stimulate the interaction of RGS18 and 14-3-3 by increasing the phosphorylation of S49. Cyclic AMP- and cyclic GMP-dependent kinases (PKA, PKG) inhibit the interaction of RGS18 and 14-3-3 by phosphorylating S216. To understand the effect of S216 phosphorylation we studied the phosphorylation kinetics of S49, S216, and S218 using Phos-tag gels and phosphorylation site-specific antibodies in transfected cells and in platelets. Cyclic nucleotide-induced detachment of 14-3-3 from RGS18 coincides initially with double phosphorylation of S216 and S218. This is followed by dephosphorylation of S49 and S218. Dephosphorylation of S49 and S218 might be mediated by protein phosphatase 1 (PP1) which is linked to RGS18 by the regulatory subunit PPP1R9B (spinophilin). We conclude that PKA and PKG induced S216 phosphorylation triggers the dephosphorylation of the 14-3-3 binding sites of RGS18 in platelets.en
dc.format.extente80251en
dc.language.isoenen
dc.relation.ispartofseriesPloS oneen
dc.relation.ispartofseries8en
dc.relation.ispartofseries11en
dc.rightsYen
dc.subjectHuman plateletsen
dc.titleCyclic nucleotide dependent dephosphorylation of regulator of G-protein signaling 18 in human platelets.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/gegenbaken
dc.identifier.rssinternalid98192en
dc.rights.ecaccessrightsopenAccess
dc.identifier.urihttp://hdl.handle.net/2262/72330


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record