dc.contributor.advisor | Porter, Richard | |
dc.contributor.author | Carroll, Audrey | |
dc.date.accessioned | 2019-05-14T15:18:17Z | |
dc.date.available | 2019-05-14T15:18:17Z | |
dc.date.issued | 2004 | |
dc.identifier.citation | Audrey Carroll, 'An investigation into the expression and physiological function of mitochondrial uncoupling proteins', [thesis], Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004, pp 267 | |
dc.identifier.other | THESIS 7419 | |
dc.description.abstract | Our study provides evidence that rat thymus contains a functioning mitochondrial uncoupling protein (UCP 1). Using reverse transcriptase-polymerase chain reaction (RT-PCR), we showed that rat thymus contains UCP 1 mRNA. Antipeptide UCP 1 antibodies, that we show to be specific for UCP 1, detected UCP 1 protein in thymus and thymus cell (i.e. thymocyte) mitochondria. We also show that
starvation (48-hour) induced no significant change in the abundance of UCP 1 in thymus and thymocyte mitochondria. Evidence for functional UCP 1 in rat thymus
mitochondria was obtained using a radiolabeled binding assay in thymus and BAT mitochondria. UCP 1 in thymus mitochondria binds [3H] GDP with similar binding
parameters, Bmax and KD values, as BAT mitochondria. In addition, a large component of the non-phosphorylating oxygen consumption by thymus mitochondria was inhibited by purine nucleotides and activated by nanomolar amounts of fatty-acids, akin to UCP 1 in BAT mitochondria. We also purified UCP 1 from thymus mitochondria by hydroxyapatite chromatography and identified the isolated protein by peptide mass mapping and tandem mass spectrometry using MALDI-TOF and LC-MS/MS, respectively. We conclude that rat thymus contains a functioning UCP 1 protein, which has the capacity to regulate metabolic flux and production and transport of reactive oxygen containing molecules in the thymus. | |
dc.format | 1 volume | |
dc.language.iso | en | |
dc.publisher | Trinity College (Dublin, Ireland). School of Biochemistry and Immunology | |
dc.relation.isversionof | http://stella.catalogue.tcd.ie/iii/encore/record/C__Rb12388008 | |
dc.subject | Biochemistry, Ph.D. | |
dc.subject | Ph.D. Trinity College Dublin | |
dc.title | An investigation into the expression and physiological function of mitochondrial uncoupling proteins | |
dc.type | thesis | |
dc.type.supercollection | thesis_dissertations | |
dc.type.supercollection | refereed_publications | |
dc.type.qualificationlevel | Doctoral | |
dc.type.qualificationname | Doctor of Philosophy (Ph.D.) | |
dc.rights.ecaccessrights | openAccess | |
dc.format.extentpagination | pp 267 | |
dc.description.note | TARA (Trinity's Access to Research Archive) has a robust takedown policy. Please contact us if you have any concerns: rssadmin@tcd.ie | |
dc.identifier.uri | http://hdl.handle.net/2262/86803 | |