| dc.contributor.author | Kelly, Daniel | |
| dc.contributor.author | O'Brien, Katie L. | |
| dc.date.accessioned | 2020-01-21T12:49:27Z | |
| dc.date.available | 2020-01-21T12:49:27Z | |
| dc.date.created | 2019 | en |
| dc.date.issued | 2019 | |
| dc.date.submitted | 2019 | en |
| dc.identifier.citation | O'Brien, K.L. & Kelly, D., Hypoxia mimicking hydrogels to regulate the fate of transplanted stem cells, 2019, Acta Biomaterialia, 88 | en |
| dc.identifier.other | Y | |
| dc.description | PUBLISHED | en |
| dc.description.abstract | Controlling the phenotype of transplanted stem cells is integral to ensuring their therapeutic
efficacy. Hypoxia is a known regulator of stem cell fate, the effects of which can be mimicked
using hypoxia-inducible factor (HIF) prolyl hydroxylase inhibitors such as
dimethyloxalylglycine (DMOG). By releasing DMOG from mesenchymal stem cell (MSC)
laden alginate hydrogels, it is possible to stabilize HIF-1α and enhance its nuclear localization.
This correlated with enhanced chondrogenesis and a reduction in the expression of markers
associated with chondrocyte hypertrophy, as well as increased SMAD 2/3 nuclear localization in
the encapsulated MSCs. In vivo, DMOG delivery significantly reduced mineralisation of the
proteoglycan-rich cartilaginous tissue generated by MSCs within alginate hydrogels loaded with
TGF-β3 and BMP-2. Together these findings point to the potential of hypoxia mimicking
hydrogels to control the fate of stem cells following their implantation into the body | en |
| dc.format.extent | 314 | en |
| dc.format.extent | 324 | en |
| dc.language.iso | en | en |
| dc.relation.ispartofseries | Acta Biomaterialia; | |
| dc.relation.ispartofseries | 88; | |
| dc.rights | Y | en |
| dc.subject | Transplanted stem cells | en |
| dc.subject | Mesenchymal stem cells | en |
| dc.subject | Hydrogel | en |
| dc.subject | Stem cell fate | en |
| dc.subject | DMOG | en |
| dc.subject | Oxygen tension | en |
| dc.subject | Hypoxia | en |
| dc.subject | Chondrogenesis | en |
| dc.subject | Hypertrophy | en |
| dc.title | Hypoxia mimicking hydrogels to regulate the fate of transplanted stem cells | en |
| dc.type | Journal Article | en |
| dc.type.supercollection | scholarly_publications | en |
| dc.type.supercollection | refereed_publications | en |
| dc.identifier.peoplefinderurl | http://people.tcd.ie/kellyd9 | |
| dc.identifier.rssinternalid | 204466 | |
| dc.identifier.doi | http://dx.doi.org/10.1016/j.actbio.2019.02.042 | |
| dc.rights.ecaccessrights | openAccess | |
| dc.identifier.orcid_id | 0000-0003-4091-0992 | |
| dc.contributor.sponsor | Science Foundation Ireland | en |
| dc.contributor.sponsorGrantNumber | 12/US/I2489 | en |
| dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1742706119301552?via%3Dihub | |
| dc.identifier.uri | http://hdl.handle.net/2262/91352 | |
