dc.contributor.author | Coleman, David | en |
dc.contributor.author | Kearney, Aoife | en |
dc.contributor.author | Kinnevey, Peter | en |
dc.date.accessioned | 2021-09-20T15:59:23Z | |
dc.date.available | 2021-09-20T15:59:23Z | |
dc.date.issued | 2021 | en |
dc.date.submitted | 2021 | en |
dc.identifier.citation | KINNEVEY PM, KEARNEY A, SHORE AC, EARLS ME, BRENNAN G, POOVELIKUNNEL TT, HUMPHREYS H, COLEMAN DC, METICILLIN-RSISTANT Staphylococcus aureus TRANSMISSION AMONG HEALTHCARE WORKERS, PATIENTS AND THE ENVIRONMENT IN A LARGE ACUTE HOSPITAL UNDER NON-OUTBREAK CONDITIONS INVESTIGATED BY WHOLE-GENOME SEQUENCING, JOURNAL OF HOSPITAL INFECTION, 118, December, 2021, 99 - 107 | en |
dc.identifier.other | Y | en |
dc.description | PUBLISHED | en |
dc.description | 21st August 2021; Epub ahead of print | en |
dc.description.abstract | Background: The role of meticillin-resistant Staphylococcus aureus (MRSA) colonization of healthcare workers (HCWs), patients and the hospital environment in MRSA transmission in non-outbreak settings is poorly understood.
Aims: To investigate transmission events (TEs) involving HCWs, patients and the environment under non-outbreak conditions in a hospital with a history of endemic MRSA using whole-genome sequencing (WGS).
Methods: HCW (N = 326) and patient (N = 388) volunteers on nine wards were tested for nasal and oral MRSA colonization over two years. Near-patient environment (N = 1164), high-frequency touch sites (N = 810) and air (N = 445) samples were screened for MRSA. Representative MRSA and clinical isolates were analysed by WGS and core-genome multi-locus sequence typing (cgMLST). Closely related isolates (≤24 allelic differences) were segregated into related isolated groups (RIGs).
Findings: In total, 155 MRSA were recovered: clinical isolates (N = 41), HCWs (N = 22), patients (N = 37), environmental isolates (N = 55). Nine clonal complexes (CCs) were identified among 110/155 MRSA sequenced with 77/110 assigned to CC22. Seventy-nine MRSA segregated into 17 RIGs. Numerous potential TEs were associated with CC22-MRSA (RIGs 1–15), CC45-MRSA (RIG-16) and CC8-MRSA (RIG-17). RIG-1, (the largest RIG) contained 24 ST22-MRSA-IVh from six HCWs, six patients, four clinical and eight environmental samples recovered over 17 months involving 7/9 wards. TEs involving HCW-to-patient, HCW-to-HCW, patient-to-patient and environmental contamination by HCW/patient isolates were evident. HCW, patient, clinical and environmental isolates were identified in four, nine, seven and 13 RIGs, respectively, with 12/13 of these containing isolates closely related to HCW and/or patient isolates.
Conclusions: WGS detected numerous potential hospital MRSA TEs involving HCWs, patients and the environment under non-outbreak conditions. | en |
dc.format.extent | 99 | en |
dc.format.extent | 107 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | JOURNAL OF HOSPITAL INFECTION | en |
dc.relation.ispartofseries | 118 | en |
dc.relation.ispartofseries | December | en |
dc.rights | Y | en |
dc.subject | MRSA | en |
dc.subject | Non-outbreak conditions | en |
dc.subject | Environmental contamination | en |
dc.subject | MRSA colonization | en |
dc.subject | Whole-genome sequencing | en |
dc.subject | Hospital transmission | en |
dc.title | METICILLIN-RSISTANT Staphylococcus aureus TRANSMISSION AMONG HEALTHCARE WORKERS, PATIENTS AND THE ENVIRONMENT IN A LARGE ACUTE HOSPITAL UNDER NON-OUTBREAK CONDITIONS INVESTIGATED BY WHOLE-GENOME SEQUENCING | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/dcoleman | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/kinnevp | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/kearnea2 | en |
dc.identifier.rssinternalid | 232741 | en |
dc.identifier.doi | https://doi.org/10.1016/j.jhin.2021.08.020 | en |
dc.rights.ecaccessrights | openAccess | |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.subject.TCDTag | Genomes, Genomics | en |
dc.subject.TCDTag | HOSPITAL-ACQUIRED INFECTION | en |
dc.subject.TCDTag | MRSA | en |
dc.subject.TCDTag | MRSA Transmission from healthcare workers to patients | en |
dc.subject.TCDTag | MRSA colonised healthcare workers | en |
dc.subject.TCDTag | MRSA contamination of the hospital environment | en |
dc.subject.TCDTag | MRSA spread | en |
dc.subject.TCDTag | ST22-MRSA-IV | en |
dc.subject.TCDTag | whole-genome sequencing | en |
dc.identifier.rssuri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=34428508&dopt=Abstract | en |
dc.identifier.orcid_id | 0000-0003-1797-2888 | en |
dc.subject.darat_thematic | Health | en |
dc.status.accessible | N | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |
dc.contributor.sponsorGrantNumber | HRA-POR-2015-1051 | en |
dc.identifier.uri | http://hdl.handle.net/2262/97093 | |