Substituted Dipyridophenazine Complexes of Cr(III): Synthesis, Enantiomeric Resolution and Binding Interactions with Calf Thymus DNA
Citation:
S. Vasudevan, J. A. Smith, M. Wojdyla, T. McCabe, N. C. Fletcher, S. J. Quinn, and J. M. Kelly, Substituted Dipyridophenazine Complexes of Cr(III): Synthesis, Enantiomeric Resolution and Binding Interactions with Calf Thymus DNA, Dalton Transactions, 39, 2010, 3990-3998Download Item:
Abstract:
[Cr(phen)2(X2dppz)]3+ {X = H, Me, or F} have been synthesised, characterised, and
chromatographically resolved into their constituent D and K enantiomers. The DNA-binding
interactions of each of the racemic complexes were investigated, with the results of linear dichroism,
thermal denaturation, and emission quenching studies indicative of intercalative binding to CT-DNA
with a significant electrostatic contribution. UV/Vis absorption titrations suggest strong DNA binding
by each of the racemic complexes, with the methylated analogue [Cr(phen)2(Me2dppz)]3+ exhibiting the
largest equilibrium binding constant. Emission quenching and UV-Vis titrations of the enantiomers of
[Cr(phen)2(dppz)]3+ imply similar binding affinities for the D and K isomers, although significant
differences between the circular dichroism spectra of the enantiomers in the presence of DNA connote
differences in binding orientation and/or conformation between the two.
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http://people.tcd.ie/jmkellyDescription:
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Author: KELLY, JOHN MOFFAT
Type of material:
Journal ArticleSeries/Report no:
Dalton Transactions39
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D and K enantiomersMetadata
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