dc.contributor.author | O'NEILL, LUKE | en |
dc.contributor.author | MCCOY, CLAIRE | en |
dc.contributor.author | QUINN, SUSAN | en |
dc.date.accessioned | 2014-03-26T10:25:55Z | |
dc.date.available | 2014-03-26T10:25:55Z | |
dc.date.issued | 2014 | en |
dc.date.submitted | 2014 | en |
dc.identifier.citation | Quinn SR, Mangan NE, Caffrey BE, Gantier MP, Williams BR, Hertzog PJ, McCoy CE, O'Neill LA., The role of Ets2 transcription factor in the induction of microRNA-155 by LPS, and its targeting by IL-10., The Journel of Biological Chemistry, 289, 9, 2014, 4316-4325 | en |
dc.identifier.other | Y | en |
dc.description | PUBLISHED | en |
dc.description.abstract | MicroRNA-155 (miR-155) is highly
expressed in many cancers such as B cell
lymphomas and myeloid leukaemia, and
inflammatory disorders such as
rheumatoid arthritis, atopic dermatitis
and multiple sclerosis. The role of miR-
155 as both a promoter of inflammation
and an oncogenic agent provides a clear
need for miR-155 itself to be stringently
regulated. We therefore investigated the
transcriptional regulation of miR-155 in
response to the respective pro- and antiinflammatory
mediators LPS and IL-10.
Bioinformatic analysis revealed Ets
binding sites on the miR-155 promoter,
and we found that Ets2 is critical for miR-
155 induction by LPS. Truncation and
mutational analysis of the miR-155
promoter confirmed the role of the Ets2
binding site proximal to the transcription
start site for LPS responsiveness. We
observed increased binding of Ets2 to the
miR-155 promoter and Ets2 deficient
mice displayed decreased induction of
miR-155 in response to LPS. IL-10
inhibited the induction of Ets2 mRNA
and protein by LPS, thereby decreasing
Ets2 function on the pri-155 promoter.
We have thus identified Ets2 as a key
novel regulator in both the positive and
negative control of miR-155 in the
inflammatory response. | en |
dc.description.sponsorship | Science Foundation Ireland (SFI), Health Research Board (HRB)/Marie Curie CoFund, and
the European Research Council (ERC) are gratefully acknowledged for funding this work
based in Ireland. Monash Institute of Medical Research funding is by the Australian National
Health and Medical Research Council, Australian Research Council, HRB/Marie Curie
CoFund, and the Victorian Government's Operational Infrastructure Support Program. We
would also like to acknowledge Erik Flemington for providing Pri-155 promoter constructs. | en |
dc.format.extent | 4316-4325 | en |
dc.language.iso | en | en |
dc.relation.ispartofseries | The Journel of Biological Chemistry | en |
dc.relation.ispartofseries | 289 | en |
dc.relation.ispartofseries | 9 | en |
dc.rights | Y | en |
dc.subject | MicroRNA-155 | en |
dc.title | The role of Ets2 transcription factor in the induction of microRNA-155 by LPS, and its targeting by IL-10. | en |
dc.type | Journal Article | en |
dc.type.supercollection | scholarly_publications | en |
dc.type.supercollection | refereed_publications | en |
dc.identifier.peoplefinderurl | http://people.tcd.ie/laoneill | en |
dc.identifier.rssinternalid | 91284 | en |
dc.identifier.doi | http://dx.doi.org/10.1074/jbc.M113.522730 | en |
dc.rights.ecaccessrights | OpenAccess | |
dc.subject.TCDTheme | Immunology, Inflammation & Infection | en |
dc.contributor.sponsor | Australian Research Council (ARC) | en |
dc.contributor.sponsor | Science Foundation Ireland (SFI) | en |
dc.contributor.sponsor | European Research Council (ERC) | en |
dc.contributor.sponsor | Health Research Board (HRB) | en |
dc.identifier.uri | http://hdl.handle.net/2262/68353 | |