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dc.contributor.authorRAMASWAMI, MANIen
dc.date.accessioned2014-07-08T09:34:50Z
dc.date.available2014-07-08T09:34:50Z
dc.date.issued2013en
dc.date.submitted2013en
dc.identifier.citationSadanandappa, MK, Redondo, BB, Michels, B, Rodrigues, V, Gerber, B, VijayRaghavan, K, Buchner, E, Ramaswami, M, Synapsin Function in GABA-ergic Interneurons Is Required for Short-Term Olfactory Habituation, JOURNAL OF NEUROSCIENCE, 33, 42, 2013, 16576-16585en
dc.identifier.issn0270-6474en
dc.identifier.otherYen
dc.descriptionPUBLISHEDen
dc.description.abstractIn Drosophila, short-term (STH) and long-term habituation (LTH) of olfactory avoidance behavior are believed to arise from the selective potentiation of GABAergic synapses between multiglomerular local circuit interneurons (LNs) and projection neurons in the antennal lobe. However, the underlying mechanisms remain poorly understood. Here, we show that synapsin (syn) function is necessary for STH and that syn(97)-null mutant defects in STH can be rescued by syn(+) cDNA expression solely in the LN1 subset of GABAergic local interneurons. As synapsin is a synaptic vesicle-clustering phosphoprotein, these observations identify a presynaptic mechanism for STH as well as the inhibitory interneurons in which this mechanism is deployed. Serine residues 6 and/or 533, potential kinase target sites of synapsin, are necessary for synapsin function suggesting that synapsin phosphorylation is essential for STH. Consistently, biochemical analyses using a phospho-synapsin-specific antiserum show that synapsin is a target of Ca(2+) calmodulin-dependent kinase II (CaMKII) phosphorylation in vivo. Additional behavioral and genetic observations demonstrate that CaMKII function is necessary in LNs for STH. Together, these data support a model in which CaMKII-mediated synapsin phosphorylation in LNs induces synaptic vesicle mobilization and thereby presynaptic facilitation of GABA release that underlies olfactory STH. Finally, the striking observation that LTH occurs normally in syn(97) mutants indicates that signaling pathways for STH and LTH diverge upstream of synapsin function in GABAergic interneurons.en
dc.format.extent16576-16585en
dc.language.isoenen
dc.relation.ispartofseriesJOURNAL OF NEUROSCIENCEen
dc.relation.ispartofseries33en
dc.relation.ispartofseries42en
dc.rightsYen
dc.subjectGeneticsen
dc.titleSynapsin Function in GABA-ergic Interneurons Is Required for Short-Term Olfactory Habituationen
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/ramaswamen
dc.identifier.rssinternalid95081en
dc.identifier.doihttp://dx.doi.org/10.1523/JNEUROSCI.3142-13.2013en
dc.rights.ecaccessrightsopenAccess
dc.identifier.urihttp://hdl.handle.net/2262/70312


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