An infection-relevant transcriptomic compendium for Salmonella enterica Serovar Typhimurium.
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2013Access:
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Kr?ger C, Colgan A, Srikumar S, H?ndler K, Sivasankaran SK, Hammarl?f DL, Canals R, Grissom JE, Conway T, Hokamp K, Hinton JC, An infection-relevant transcriptomic compendium for Salmonella enterica Serovar Typhimurium., Cell host & microbe, 14, 6, 2013, 683-95Download Item:
Abstract:
Bacterial transcriptional networks consist of hundreds of transcription factors and thousands of promoters. However, the true complexity of transcription in a bacterial pathogen and the effect of the environments encountered during infection remain to be established. We present a simplified approach for global promoter identification in bacteria using RNA-seq-based transcriptomic analyses of 22 distinct infection-relevant environmental conditions. Individual RNA samples were combined to identify most of the 3,838 Salmonella enterica serovar Typhimurium promoters in just two RNA-seq runs. Individual in vitro conditions stimulated characteristic transcriptional signatures, and the suite of 22 conditions induced transcription of 86% of all S. Typhimurium genes. We highlight the environmental conditions that induce the Salmonella pathogenicity islands and present a small RNA expression landscape of 280 sRNAs. This publicly available compendium of environmentally controlled expression of every transcriptional feature of S. Typhimurium constitutes a useful resource for the bacterial research community.
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http://people.tcd.ie/kahokamphttp://people.tcd.ie/krogerc
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PUBLISHED
Author: HOKAMP, KARSTEN; KROGER, CARSTEN
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Cell host & microbe14
6
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GeneticsSubject (TCD):
Genes & SocietyDOI:
http://dx.doi.org/10.1016/j.chom.2013.11.010ISSN:
1931-3128Metadata
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