Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography
Citation:
Weierstall, U., James, D., Wang, C., (...), Caffrey, M., Cherezov, V., Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography, Nature Communications, 5, 2014, 3309-Download Item:
Abstract:
Lipidic cubic phase (LCP) crystallization has proven successful for high-resolution structure determination of challenging membrane proteins. Here we present a technique for extruding gel-like LCP with embedded membrane protein microcrystals, providing a continuously renewed source of material for serial femtosecond crystallography. Data collected from sub-10-μm-sized crystals produced with less than 0.5 mg of purified protein yield structural insights regarding cyclopamine binding to the Smoothened receptor.
Sponsor
Grant Number
National Science Foundation (NSF)
MCB 1120997
National Science Foundation (NSF)
MCB 1021557
Author's Homepage:
http://people.tcd.ie/mcaffreDescription:
PUBLISHED
Author: CAFFREY, MARTIN
Sponsor:
National Science Foundation (NSF)National Science Foundation (NSF)
Type of material:
Journal ArticleCollections
Series/Report no:
Nature Communications5
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Lipidic cubic phase (LCP)DOI:
http://dx.doi.org/10.1038/ncomms4309Metadata
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