Browsing Clinical Microbiology (Theses and Dissertations) by Date of Publication
Now showing items 1-20 of 28
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The molecular characterization of the clumping Factor A (ClfA) from Staphylococcus aureus using monoclonal antibodies
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2000)Clumping factor (ClfA) of Staphylococcus aureus is the major fibrinogen- binding adhesin located on the cell surface of the bacterium. Monoclonal antibodies (mAbs) were produced to two recombinant truncated ClfA proteins, ... -
Characterisation of the Elastin Binding Protein (EbpS) of Staphylococcus aureus
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2001)Staphylococcus aureus expresses an array of surface proteins that promote interaction of the bacterium with the host. Some surface proteins promote binding to components of the host extracellular matrix and can act as ... -
Analysis and development of the recombinant Semliki Forest virus vector as a cancer gene therapy agent
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2001)Increased understanding of the molecular pathogenesis of cancer has shown that its accumulation is the result of multiple genetic alterations. Cancer is a disease of altered genes, with the most predominantly altered ... -
Plasmodium falciparum aminopeptidases and their role in haemoglobin degradation in malaria-infected erthrocytes
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2001)Malaria remains one of the world's most important infectious diseases. There is no vaccine available, and the spread of drug resistance has narrowed the choice of chemotherapy, especially for the most lethal human malaria ... -
StpA and the regulation of OmpF porin expression in Escherichia coli
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2001)When the proteomic profile of a wild-type strain of Escherichia coli and its stpA hns mutant derivative were compared by two-dimensional gel electrophoresis, the levels of expression of several proteins were altered. One ... -
A role for proteases and transcriptional regulation in the expression of ClfB on the surface of staphylococcus aureus
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2002)Staphylococcus aureus is an important human pathogen. This bacterium expresses proteins on its surface capable of binding to many host ligands including fibrinogen, fibronectin, collagen, elastin and von Willebrand factor. ... -
The role of beta-lactamase in low-level cephalosporin-resistant serratia marcescens
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2002)In enterobacteria, high-level cephalosporin resistance is commonly mediated by constitutive over-production or derepression of chromosomal AmpC β-lactamase. This derepression is frequently associated with mutations in ... -
Molecular characterisation of the interactions between staphylococcus aureus and elastin
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2002)Previous studies have shown that a cell-surface 83 kDa elastin-binding protein of Staphylococcus aureus (EbpS) mediates binding to soluble elastin. Antibodies were produced to the N terminus and C terminus of EbpS. Western ... -
Investigation of the pathogenicity of Rubella virus for the central nervous system
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2002)Rubella virus (RV) is the cause of a mild self limiting disease, known as German measles, which predominates during childhood. It is however the devastating teratogenic effects of this virus that are of most concern. Rare ... -
Regulation of Antigen 43, a phase-variable autoaggregation factor of Escherichia coli
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2003)The Antigen 43 protein (Ag43) of Escherichia coli, encoded by the agn43 gene, has previously been shown to be expressed in a phase-variable manner. A plasmidborne fusion of the agn43 regulatory region to the reporter gene ... -
Functional analysis of the VirB protein of Shigella flexneri
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2003)The VirB protein is a key regulator of virulence gene expression in Shigella flexneri, a facultative enteroinvasive pathogen that causes bacillary dysentery. Genetic evidence has shown that VirB is required for the activation ... -
The control of DNA gyrase expression and its role in deletion formation in Salmonella enterica
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2003)Salmonella typhimurium strain CJD671 can undergo the deletion of a 5kb segment of DNA from its large virulence plasmid. This results in a transcriptional fusion between the rlgA gene, encoding a putative site-specific ... -
Development of the recombinant Semliki Forest virus vector as a gene therapy agent for the central nervous system
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2004)Multiple sclerosis (MS) is a neurological disease of the central nervous system (CNS), and a major cause of disability in young adults. It is characterized by the presence of inflammatory infiltrates containing autoreactive ... -
Inhibition of K-BALB murine tumours using Semliki Forest virus and its derived factor
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2005)The induction of cytopathic effects in tumour cells, often by apoptosis, is the primary goal of most non-surgical cancer therapies. Cancer gene therapy represents a variety of potentially therapeutic strategies involving ... -
Investigation of reduced susceptibility to glycopeptides among meticillin-resistant Staphylococcus aureus recovered in Ireland (1998-2004)
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2009)Meticillin-resistant Staphylococcus aureus (MRSA) isolates (n=3,189) from 2990 patients were studied by agar screening and the E-test™ macromethod to investigate reduced susceptibility to glycopeptides among six collections ... -
Antimicrobial resistance and virulence in Escherichia coli bloodstream isolates : a phenotypic, genetic and genomic perspective
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2010)Extraintestinal pathogenic Escherichia coli (ExPEC) are a major cause of sepsis, with a high associated mortality. They are widely reported to be the most frequent Gram-negative bacterial pathogen recovered from bacteraemia ... -
Molecular characterisation of carbapenem resistance of Acinetobacter species in an Irish tertiary care hospital
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2010)This study was undertaken with the primary aim of characterising the molecular epidemiology and mechanisms of resistance to carbapenems in Acinetobacter isolates collected at St. James’s Hospital, Dublin. -
Antimicrobial resistance in clinical isolates of Pseudomonas aeruginosa
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2011)Pseudomonas aeruginosa is one of the most feared Gram-negative bacterial pathogens. It is not only among the most frequently isolated Gram-negative organisms in bloodstream and wound infections, pneumonia, intra-abdominal-sepis ... -
Anti-infective strategies in Pseudomonas aeruginosa biofilm formation
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2014)P. aeruginosa infection is one of the main causes of lung function decline in patients with cystic fibrosis (CF). P. aeruginosa is associated with biofilm formation which can increase the rate of resistance to antibiotic ... -
The role of the Salmonella PagN protein in adhesion and invasion
(Trinity College (Dublin, Ireland). School of Medicine. Discipline of Clinical Microbiology, 2015)Salmonella, an important genus of Gram-negative enteric bacteria, is the causative agent of many different diseases including Typhoid Fever and Gastroenteritis. Salmonella utilises multiple methods of invading mammalian ...