Biochemistry (Theses and Dissertations): Recent submissions
Now showing items 141-160 of 333
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An investigation of the link between the bacterial derived queuine molecule and tyrosine production
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)Queuine, a dietary derived 7-deazaguanine molecule, has previously been shown to prevent lethality and other symptoms in germ-free mice that were maintained on a tyrosine-free diet. This work suggested a link between queuine ... -
Structural and functional studies of Pseudomonas aeruginosa strain PAOI Lysine Specific Permease (LysP)
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2013)The means by which substrates are shuttled across prokaryotic membranes by membrane transporters and the conformational changes involved are unique to each transporter. To decipher the underlying mechanism of substrate ... -
Modulation of innate immunity by the vaccinia virus protein K7 and its target DDX3
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2010)Vaccinia virus (VACV) has many mechanisms to subvert and modulate the host immune response. One well characterised VACV protein that does this is A52. K7 was found from a search of the VACV genome looking for genes with ... -
A role for type 111 interferons in the natural killer cell immune response to virus
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Natural Killer (NK) cells are fundamental effector cells of the innate immune system that function to eliminate virally infected and transformed cells. One key way in which they do this is through the production of cytokines, ... -
Chitosan based adjuvants promote proinflammatory Th1 and Th17 responses
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Particulate vaccine adjuvants, including alum and biodegradable polymer microparticles, are strong activators of antigen-specific humoral immunity. These particulates enhance NLRP3 inflammasome activation, but they are ... -
Investigation of the oxidative folding pathway in Trypanosoma brucei
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2013)In eukaryotic organisms the native structure of proteins destined to be secreted, such as immunoglobulins, or mounted surface proteins rely on the formation of disulphide bridges between cysteine residues for structural ... -
Structural studies of myxoma virus antagonists of innate immunity
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Viral evasion of the host immune system is achieved via antagonism of antiviral signalling pathways by the expression of immunomodulating proteins. Vaccinia virus protein C7 and its homologues function as determinants ... -
Novel Peroxisome Proliferating Activated Receptor (PPAR) modulator drug discovery : consensus virtual screening and characterisation of biochemcial and pharmacological activities
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Peroxisome proliferator-activated receptors (PPARs) are ligand activated transcription factors that form a subfamily of the nuclear receptor superfamily. PPARs form heterodimers with the 9-cis-retinoic acid receptor (RXR) ... -
Structural and functional characterisation of a novel bovine β-defensin gene cluster
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2013)Antimicrobial peptides (AMPs) have been characterised by their ability to neutralise pathogens directly by disrupting their membranes. Recently described roles for these peptides include chemotactic activity, wound healing ... -
Polymorphisms in members of the Toll-like receptor signalling pathway and their role in infectious disease
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2009)Toll-like receptors (TLRs) play an important role in mediating the host's response to invading pathogens. In humans, they comprise of a family of ten pattern recognition receptors (PRRs) located on innate immune cells. ... -
An investigation of the physiological and pathological role of uncoupling proteins
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Uncoupling proteins (UCPs) are found in numerous different tissues and have been associated with many physiological and pathological conditions. After decades of research on uncoupling proteins, there is still only a modest ... -
Nutrient availability regulates Dendritic cell metabolism and function to modulate T cell responses
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2015)An emerging view in the field of immunometabolism is that cellular metabolism has a role that extends beyond simply allowing the cell to meet energy and biosynthetic demands, and represents a fundamental physiological ... -
The immunodulatory effects of the cAMP effector Epac
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Cyclic AMP is an extremely important second messenger and is known to be a central mediator of inflammation and regulator of the immune response. For many years it was thought that the effects of cAMP on immunity were ... -
Mal is not esential for TLR2 signalling and inhibits signalling by TLR3
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2010)This thesis set out to systematically analyse the role of Mai in signalling by TLR2, TLR3 and TLR4 in murine macrophages and dendritic cells. It has revealed that Mai is not essential to TLR2 signal transduction, is required ... -
Interferon lambda, dendritic cells and Hepatitis C virus infection
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2013)It is estimated that ~3% of the world's population is infected with Hepatitis C virus (HCV), a hepatotropic virus which can lead to liver fibrosis, cirrhosis and hepatocellular carcinoma. Of those infected, ~20% clear the ... -
The role of microRNA-107 in Toll-like receptor signaling
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2010)MicroRNAs (miRNAs) are a novel class of small non-coding RNA regulatory molecules which post-transcriptionally regulate genes in a fine-tuning manner. Multiple miRNAs have been shown to be regulated by Toll-like receptors ... -
Identification of the components of the eukaryotic queuine transglycosylase complex and the effect of its substrate queuine on metabolism
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)Queuine is a 7-deazaguanine derivative of guanine, which replaces guanine in position 34 of the anticodon loop of the transfer RNA for the amino acids, asparagine, aspartic acid, histidine and tyrosine. This base or related ... -
Mechanistic and functional studies on biliverdin IXa reductase from the cyanobacterium synechocystis sp.PCC6803
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2009)The enzymes of heme metabolism and phycobilin biosynthesis in the cyanobacterium Synechocystis sp. PCC6803, along with their reducing partners, ferredoxin and ferredoxin reductase were produced through recombinant DNA ... -
Exploring an immune function for murine SARM
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2012)Innate immune cells, such as tissue-residing macrophages and dendritic cells (DCs) play a key role in initiating an immune response following the detection of invading pathogens via germline-encoded pattern-recognition ... -
GlcNAc transferases and glycoengineering in Chinese hamster ovary cells
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2011)Glycosylation constitutes one of the most important post-translational modifications of proteins, with over 60% of proteins known to be glycosylated. GlcNAc transferases (GnTs) belong to a large subfamily of glycosyltransferases ...
