Biochemistry (Theses and Dissertations): Recent submissions
Now showing items 241-260 of 333
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Mechanistic studies on human liver Biliverdin-IX beta reductase
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)The reaction mechanism of human biliverdin-IXβ reductase (BVR-B) has been investigated using a number of approaches. The preference for NADPH over NADH has been examined using site-directed mutagenesis. The crystal structure ... -
Investigations into the physiological expression of UCP1 and UCP3
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Previous studies have indicated that acute exercise induces large increases in uncoupling protein 3 in skeletal muscle, whereas endurance training results in marked decreases in skeletal muscle uncoupling protein 3 at both ... -
Synthesis of chemical probes targeting membrane proteins
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Membrane proteins carry out important physiological functions, including roles as enzymes and receptors, and are the targets for 50% of current drugs on the market,1 indicating their pharmacological importance. Knowledge ... -
Inflammation and uterine disease in the postpartum dairy cow
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Following calving, inflammation of the uterus is common during the first week postpartum and is essential for the reparative process of involution. However, whilst the majority of cows resolve this inflammation a significant ... -
Adjuvants targeting innate T lymphocytes for improved oral vaccination
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Effective mucosal vaccines can provide protective local and systemic immunity. Oral vaccination in particular has the potential to offer a safer and more efficacious approach than injection-based approaches, especially ... -
Inhibitor of Apoptosis Proteins as Targets for the Treatment of Malignant Rhabdoid Tumours
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Malignant rhabdoid tumor (MRT) is a rare paediatric cancer which unfortunately is highly refractive to treatment. Retrospective reviews commonly place the survival rate at approximately 30-35 %. Thus, there is an urgent ... -
Investigating the role of specific inflammasome components during inflammatory disease
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Inflammasomes are multi-protein complexes that act as intracellular innate immune receptors. The first inflammasome was initially defined by Martinon et al in 2002 and 16 years on, this increasingly explored area has led ... -
Structure and function studies of lipoprotein signal peptidase II (LspA) from Pseudomonas aeruginosa PAO1 for antibiotic development
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)The standard and most successful treatment for bacterial infections is antibiotic therapy, with drugs such as penicillin. Antibiotics were discovered in 1928 and were successfully implemented clinically to treat bacterial ... -
INFLAMMASOME ACTIVATED IL-1β AND THE SWITCH TO PATHOLOGICAL INFLAMMATION IN THE BOVINE ENDOMETRIUM
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Inflammation of the postpartum uterus is a normal physiological event, crucial for tissue involution and repair. Immediately following parturition, the uterus experiences dysregulation of its microbiome and is exposed to ... -
The Role of Mitochondrial Uncoupling Protein 3 in T Cell Function
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Uncoupling proteins (UCPs) are members of the mitochondrial anion carrier superfamily that can mediate the transfer of protons into the mitochondrial matrix from the intermembrane space. Our laboratory has reported evidence ... -
Investigating the impact of natural killer (NK) cell metabolism on NK cell effector function
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)It is now known that metabolism, in addition to providing energy and biochemical building blocks, also regulates immune cell function. Over the last few years, we have increased our understanding of how NK cell metabolism ... -
MicroRNA-21 Limits Macrophage Responses to Mycobacterium tuberculosis
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Mycobacterium tuberculosis (Mtb) is an intracellular pathogen that subverts the innate immune response. The Mtb pandemic is of global concern. Emerging strains of antibiotic-resistant Mtb warrant the development of ... -
Harnessing the therapeutic properties of the Heme Oxygenase system
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2018)The stress response enzyme, Heme Oxygenase 1 (HO-1), has been identified as an important immunomodulator which is highly upregulated in response to cellular stress and inflammation. HO-1 catalyses the conversion of free ... -
The establishment of hybrid cell lines from human pancreas
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)The need for research into diabetes mellitus is ever increasing. Of specific interest are the mechanisms responsible for glucose-responsive insulin secretion and the autoimmune destruction of pancreatic p-cells. However, ... -
Studies on human biliverdin-IX alpha reductase and linear tetrapyrrole signaling
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Human Biliverdin-IXa reductase (BVR-A) has been cloned and overexpressed in E.coli as a GST- and Hexahistidine fusion protein. The full length cDNA encoding the enzyme has been amplified via PCR and hgated into the pGEX-KG ... -
Biochemical investigations of hyperhomocysteinemia
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Hyperhomocysteinemia, a condition where plasma homocysteine occurs at an abnormally elevated level, is a common finding in vascular disease subjects. Several genetic and nutritional factors have been implicated. The first ... -
Is Apolipoprotein-E modulated in peripheral blood mononuclear cells by IFN-β and is this to related patient responsiveness in multiple sclerosis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Multiple Sclerosis is a neurodegenerative disorder involving the attack of blood resident T-lymphocytes on the myelin sheath surrounding the neuron. This condition is particularly prevalent amongst white, northern European ... -
The role of thiols in the activation of the platelet integrin αllbβ3
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Platelets play a vital role in maintaining normal haemostasis. They circulate passively in the blood in an non-adhesive slate as discrete smooth discs. When platelets encounter a vessel wall injury or are exposed to foreign ... -
A role for microRNA-21 in the regulation of gastrointestinal health and disease
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2017)The gastrointestinal (GI) tract is a crucial site of innate and adaptive immune regulation, balancing tolerance of beneficial commensal microorganisms and reaction to invading pathgens. Pattern recognition receptors (PRRs), ... -
The role of T cell subsets in experimental autoimmune encephalomyelitis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2015)Multiple Sclerosis (MS) is a chronic, demyelinating disease which affects the central nervous system (CNS) resulting in progressive cognitive decline and physical disability. Experimental autoimmune encephalomyelitis (EAE) ...
