Biochemistry (Scholarly Publications): Recent submissions
Now showing items 341-360 of 734
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Manipulation of autophagy in phagocytes facilitates Staphylococcus aureus bloodstream infection.
(2015)The capacity for intracellular survival within phagocytes is likely a critical factor facilitating the dissemination of Staphylococcus aureus in the host. To date, the majority of work on S. aureus-phagocyte interactions ... -
MemProtMD: Automated Insertion of Membrane Protein Structures into Explicit Lipid Membranes
(2015)There has been exponential growth in the number of membrane protein structures determined. Nevertheless, these structures are usually resolved in the absence of their lipid environment. Coarse-grained molecular dynamics ... -
In meso in situ serial X-ray crystallography of soluble and membrane proteins
(2015)The lipid cubic phase (LCP) continues to grow in popularity as a medium in which to generate crystals of membrane (and soluble) proteins for high-resolution X-ray crystallographic structure determination. To date, the PDB ... -
Gating topology of the proton-coupled oligopeptide symporters
(2015)Peptide transport is the main route through which the body absorbs and retains dietary protein and hence plays an important role in human physiology (Steinhardt and Adibi, 1986). The combined action of acid hydrolysis in ... -
Respiratory infection with a bacterial pathogen attenuates CNS autoimmunity through IL-10 induction
(2015)Infection with viral or bacterial pathogens has been linked with the development of multiple sclerosis (MS), while infection with helminth parasites has been associated protection against MS and other autoimmune diseases. ... -
Coupling of lysosomal and mitochondrial membrane permeabilization in trypanolysis by APOL1.
(2015)Humans resist infection by the African parasite Trypanosoma brucei owing to the trypanolytic activity of the serum apolipoprotein L1 (APOL1). Following uptake by endocytosis in the parasite, APOL1 forms pores in ... -
Memory Th1 Cells Are Protective in Invasive Staphylococcus aureus Infection.
(2015)Mechanisms of protective immunity to Staphylococcus aureus infection in humans remain elusive. While the importance of cellular immunity has been shown in mice, T cell responses in humans have not been characterised. Using ... -
Adenosine-mono-phosphate-activated protein kinase-independent effects of metformin in T cells.
(2014)The anti-diabetic drug metformin regulates T-cell responses to immune activation and is proposed to function by regulating the energy-stress-sensing adenosine-monophosphate-activated protein kinase (AMPK). However, the ... -
MTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function
(2014)The mammalian target of rapamycin complex 1 (mTORC1) is a key regulator of cellular metabolism and also has fundamental roles in controlling immune responses. Emerging evidence suggests that these two functions of mTORC1 ... -
Metabolic reprograming in macrophage polarization.
(2014)Studying the metabolism of immune cells in recent years has emphasized the tight link existing between the metabolic state and the phenotype of these cells. Macrophages in particular are a good example of this phenomenon. ... -
RNA-seq Transcriptional Profiling of Peripheral Blood Leukocytes from Cattle Infected with Mycobacterium bovis.
(2014)Bovine tuberculosis, caused by infection with Mycobacterium bovis, is a major endemic disease affecting cattle populations worldwide, despite the implementation of stringent surveillance and control programs in many ... -
TRAM is required for TLR2 endosomal signaling to type I IFN induction.
(2014)Detection of microbes by TLRs on the plasma membrane leads to the induction of pro-inflammatory cytokines such as TNF-α, via activation of NF-κB. Alternatively, activation of endosomal TLRs leads to the induction of type ... -
SARM regulates CCL5 production in macrophages by promoting the recruitment of transcription factors and RNA polymerase II to the Ccl5 promoter.
(2014)The four Toll/IL-1R domain-containing adaptor proteins MyD88, MAL, TRIF, and TRAM are well established as essential mediators of TLR signaling and gene induction following microbial detection. In contrast, the function of ... -
TrypanoCyc: a community-led biochemical pathways database for Trypanosoma brucei.
(2015)The metabolic network of a cell represents the catabolic and anabolic reactions that interconvert small molecules (metabolites) through the activity of enzymes, transporters and non-catalyzed chemical reactions. Our ... -
Structural basis for ion selectivity revealed by high-resolution crystal structure of Mg2+ channel MgtE
(2014)Magnesium is the most abundant divalent cation in living cells and is crucial to several biological processes. MgtE is a Mg 2 þ channel distributed in all domains of life that contributes to the maintenance ... -
A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes
(2015)The lipid cubic phase or in meso method is a robust approach for crystallizing membrane proteins for structure determination. The uptake of the method is such that it is experiencing what can only be described ... -
A conformational landscape for alginate secretion across the outer membrane of Pseudomonas aeruginosa
(2014)The exopolysaccharide alginate is an important component of biofilms produced by Pseudomonas aeruginosa , a major pathogen that contributes to the demise of cystic fibrosis patients. Alginate exits the cell via the ... -
Renaturing membrane proteins in the lipid cubic phase, a nanoporous membrane mimetic
(2014)Membrane proteins play vital roles in the life of the cell and are important therapeutic targets. Producing them in large quantities, pure and fully functional is a major challenge. Many promising projects end when intractable ... -
The lipid cubic phase or in meso method for crystallizing proteins. Bushings for better manual dispensing
(2014)The lipid cubic phase or in meso method can be used to set up crystallization trials of soluble and membrane proteins. The cubic phase is noted for being a sticky and viscous mesophase. Dispensing the protein-laden ... -
Experimental phasing for structure determination using membrane-protein crystals grown by the lipid cubic phase method
(2015)Despite the marked increase in the number of membrane- protein structures solved using crystals grown by the lipid cubic phase or in meso method, only ten have been determined by SAD/MAD. This is likely to be ...
