RSS 1.0Microbiology (Theses and Dissertations)
Recent Submissions
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Understanding how community context drives virulence-associated traits in the Cystic Fibrosis pathogen Pseudomonas aeruginosa
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2025)The lungs of patients with Cystic Fibrosis (CF) are often chronically colonised by a plethora of microbial species, including the major CF pathogen Pseudomonas aeruginosa (P. aeruginosa). Herewith, when examining ... -
Investigating the Impact of Anthropogenic Change on Soil Microbiome Functioning and Crop Health
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2025)Anthropogenic change poses significant challenges for society today. Soil microbial communities are under constant anthropogenic stress from pollution, rising climates, pesticide use, and deforestation to name a few. We ... -
Characterising the small RNA-regulatory architecture of Acinetobacter baumannii
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2025)The Gram-negative bacterium Acinetobacter baumannii is a significant opportunistic pathogen in critically ill and hospitalised patients. It exhibits a remarkable ability to survive under harsh environmental conditions, ... -
Characterisation of the alternative sigma factor SigAB in Acinetobacter baumannii
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2024)Acinetobacter baumannii is a priority pathogen and is known to be a leading cause of nosocomial infections worldwide. It is associated with a variety of illnesses including septicaemia, pneumonia, and urinary tract ... -
Molecular characterisation of tagO-deficient mutants of Staphylococcus aureus
(Trinity College (Dublin, Ireland). Department of Microbiology, 2017)Wall teichoic acid (W TA) is a major component of the Gram -positive bacterial cell wall. Despite not being essential, it plays important roles in cell growth, division, morphology and virulence. The enzyme TagO initiates ... -
Analysis of the mechanism of protein A release and its contribution to immune evasion by Staphylococcus aureus
(Trinity College (Dublin, Ireland). Department of Microbiology, 2016)The immunoglobulin binding protein A (SpA) of Staphylococcus aureus was one of the first cell wall-associated proteins of Gram-positive bacteria to be identified and characterised. It is synthesized as a precursor with a ... -
Investigating the contributions to cell function of the different Swi-Snf complex subunits in Saccharomyces cerevisiae
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2024)The eukaryotic genome is packaged as a DNA-protein structure known as chromatin. The basic subunit of chromatin is the nucleosome, which contains two copies of each of the core histone proteins H2A, H2B, H3, and H4, around ... -
Investigating a role for linker histone H1 in quiescent cells of Saccharomyces cerevisiae
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2024)The aim of this study was to determine whether either Hho1p or Hmo1p can be considered the best candidate to function as linker histone H1 in Saccharomyces cerevisiae. The hypothesis was that yeast linker histone H1 would ... -
Understanding Tolerance to Biocides and its Consequences in Clinical Isolates of Klebsiella pneumoniae - How to Treat an ESKAPE Pathogen
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2024)Biocides such as Triclosan, Benzalkonium Chloride, and Chlorhexidine have been used as disinfectants for many years, however the effects of their use and the subsequent selective pressures applied to microbial populations ... -
The transcriptional landscape and small RNAs in Acinetobacter baumannii AB5075 during antibiotic and environmental stress.
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2024)Multi-drug resistant Acinetobacter baumannii is considered a major challenge because it causes a variety of serious infections. Studying these bacteria at the level of their genetic response could facilitate finding a way ... -
Understanding benzalkonium chloride tolerance and its implications in antibiotic resistance and other phenotypes in clinical isolates of Klebsiella pneumoniae
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2024)In this study, clinical isolates of Klebsiella pneumoniae, a Gram-negative pathogen associated with hospital acquired infections, were tested for both phenotypic and genotypic changes following adaptation to the commonly ... -
Investigating the potential to source novel postbiotics with anti-microbial or immune-modulatory activity from distillery waste
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2024)The purpose of this project is to find a novel source for antimicrobial and immunomodulatory compounds. Here we investigate the antimicrobial and immunomodulatory potential of postbiotics sourced from whisky distillation. ... -
Preventing protein-dependent biofilm formation in Staphylococcus aureus by targeting the serine aspartate repeat protein C and fibronectin binding proteins
(Trinity College (Dublin, Ireland). Department of Microbiology, 2017)Staphylococcus aureus is a leading cause of biofilm infections on indwelling medical devices. S. aureus biofilm infections are intrinsically difficult to treat. They are recalcitrant to conventional antibiotics and resistant ... -
Understanding the Role of Host Factors in the Modulation of Virulence Factors of Staphylococcus aureus.
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023)Staphylococcus aureus is adept at colonising and infecting the human host. Host factors are known to influence pathogen physiology and this thesis aimed to examine the effects of human bile and human plasmin on S. aureus. ... -
Distinct phenotypes and transcriptomes of tup1 and cyc8 mutants suggest novel roles for TUP1 and CYC8 in Saccharomyces cerevisiae
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023)The Tup1-Cyc8 (Ssn6) co-repressor complex is a regulator of gene transcription in the yeast Saccharomyces cerevisiae. This project provides insights into the distinct roles of Tup1p and Cyc8p in gene repression, identifies ... -
Investigating Tup1-Cyc8 complex function in Saccharomyces cerevisiae following the confirmation and characterization of a TUP1 conditional mutant
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023)The Tup1-Cyc8 complex is a well-defined corepressor complex found in S. cerevisiae. It is known to regulate close to 3% of all yeast genes and has orthologs reported in mammals. Its exact functioning is not well-understood. ... -
Genetic and Molecular Studies of Virulence Factors of Staphylococcus aureus
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023) -
Investigation of the cellular location of Multicopper Oxidase and its contribution to copper tolerance and immune resistance in Staphylococcus aureus
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2022)In high amounts copper exerts toxic effects on bacteria, and therefore its cellular concentrations must be tightly controlled. Staphylococcus aureus is a WHO priority pathogen responsible of an enormous burden on healthcare ... -
Accelerated Evolution of Lager Yeast Strains for Improved Flavour Profiles
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2022)S. pastorianus is an interspecific hybrid resulting from natural hybridization between S. cerevisiae and S. eubayanus. These two species belong to the Saccharomyces genus, a genus that encompasses different species related ... -
Drug Repurposing as a Strategy to Uncover Effective Antibacterial Compounds using Salmonella as a model
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)Without doubt, antimicrobial resistance (AMR) is one of the most serious global challenge for public, animals and environmental health. Tackling this huge issue that is threatening modern medicine, is an urgent priority ...
