Genetics (Theses and Dissertations): Recent submissions
Now showing items 61-80 of 218
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An Exploration of Mitochondrially Targeted Gene Therapies for Ocular Disorders
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Genetics, 2020)Mitochondria play a vital role in numerous fundamental processes of the cell such as ATP synthesis, cellular signalling, reactive oxygen species production, calcium regulation and apoptosis to name but a few. Given their ... -
Blood-brain barrier response in the context of sports-related TBI
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Genetics, 2020)Traumatic brain injury (TBI) is one of the largest causes of mortality and disability globally. The severity of TBI is clinically categorised into mild, moderate and severe injuries. Moderate and severe TBI often present ... -
Iron regulated surface determinants of Staphylococcus aureus and S. lugdunensis : their roles in pathogen-host interactions
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)Staphylococcus aureus is a human pathogen that causes invasive infections. The ability to internalize into and persist within host cells is thought to contribute to pathogenesis. The study described here has identified a ... -
Dissecting the functional link between transcription factor codes and axon guidance genes in Drosophila motor neurons
(Trinity College (Dublin, Ireland). Department of Genetics, 2013)As axons navigate through the extracellular environment they are exposed to different guidance cues that will steer them when sensed by receptors and cell adhesion molecules [CAMS) present on their membranes. The intricate ... -
A functional genomics approach to study plant reproductive development
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)A fundamental question in developmental biology is how differentiated cell types and organs are formed from undifferentiated precursor cells. The establishment of the body plan of an organism is ultimately defined by its ... -
The evolution of molecular chaperones and their clients
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)Molecular chaperones are ancient and ubiquitous proteins that help other proteins in the cell to fold. In this thesis, we investigate the evolution of assisted protein folding from two perspectives: the evolution of the ... -
Caspase and granzyme regulated events in apoptosis and inflammation
(Trinity College (Dublin, Ireland). Department of Genetics, 2011)The caspase and granzyme proteases are key regulators of both programmed cell death (apoptosis) and inflammation. They achieve this regulation through the limited proteolysis of protein substrates, this has the consequence ... -
Unravelling the evolutionary complexity of RNA viruses
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2009)Viruses are a delicate group of organisms renowned for the serious diseases they inflict. They affect all cellular life and are subdivided into RNA-based and DNA-based viruses depending on the type of genomic nucleotide ... -
Expression of antibody fragments in tobacco using transient and stable expression systems
(Trinity College (Dublin, Ireland). Department of Genetics, 2011)Plants are an attractive alternative to mammalian and microbial cell culture systems as a potential production platform for recombinant proteins. Apart from avoiding ethical problems associated with transgenic animals, ... -
Next generation sequencing of ancient DNA
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)The application of next generation sequencing (NGS) technologies into the field of ancient DNA has seen a paradime shift in the level of analysis that can be completed using archaeological samples. However, to fully access ... -
Surface proteins and restriction-modification systems of bovine mastitis strains of Staphylococcus aureus
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)Staphylococcus aureus is frequently associated with bovine mastitis, one of the most economically important diseases in the dairy industry. The bovine S. aureus strain RF122 represents a geographically widespread clone ... -
Population genetics of modern and ancient cattle
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)The domestication of cattle was a milestone in human history. It is widely accepted that cattle were domesticated in at least two independent domestication events; the Fertile Crescent was the primary domestication centre ... -
Characterisation of the second binding protein of immunoglobulin (SBi) in Straphylococcus aureus
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)The second binding protein for immunoglobulin (Sbi) of Staphylococcus aureus comprises two IgG binding domains (D1 and D2) that share 30% sequence homology with the IgG binding domains of protein A (Spa) and two domains ... -
Investigation of the regulation of Bcl-2 family members in apoptosis and cancer
(Trinity College (Dublin, Ireland). Department of Genetics, 2010)Apoptosis is a highly controlled mechanism of cell suicide that is activated in response to cellular stress and damage. The Bcl-2 family of proteins play a key role in the regulation of this process. Opposing subsets of ... -
Specification of floral reproductive organs : the role of AGAMOUS
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)Homeosis is referred to as the transformation of an organ's identity toward the identity of another organ. This term was coined in 1894 and soon thereafter; these organ transformations were attributed to the variation in ... -
Analysis of the gene regulatory network underlying reproductive floral organ development through gene perturbation experiments
(Trinity College (Dublin, Ireland). Department of Genetics, 2013)Homeosis or homeotic transformation refers to the formation of one body structure or organ in place of another. For more than two decades, the floral homeotic genes have been at the center of intense studies that showed ... -
Defining the role of the AP2/ERF transcription factors Rap2.4 and Rap2.4b in stress responses in the model plant Arabidopsis thaliana
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2010)This study characterises the molecular properties and the function of two Arahidopsis AP2/ERF transcription factors, Rap2.4 and Rap2.4b. The two genes belong to the DREB subgroup, members of which have been demonstrated ... -
Evolution of the OmpR regulon in Salmonella enterica serovar Typhimurium and Escherichia coli in response to low pH
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)Two-component systems (TCS) enable bacteria to sense, respond and adapt to a wide range of environmental stimuli. The OmpR/EnvZ TCS is composed of the inner membrane sensor kinase EnvZ and the response regulator OmpR. In ... -
Automated annotation of yeast genomes
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)I have built an automated annotation pipeline (named YGAP - Yeast Genome Annotation Pipeline), designed specifically for new yeast genome sequences lacking transcriptome data. YGAP uses homology and synteny information ... -
Picking the high hanging fruit : automated ways to annotate awkward genes
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)In Chapter 2 I describe the development of software called SearchDOGS (Database of Orthologous Genomic Segments). By identifying regions of conserved local synteny across species using the synteny information contained in ...
