Genetics (Theses and Dissertations): Recent submissions
Now showing items 81-100 of 218
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Control of conjugation in the plasmid pSf-R27
(Trinity College (Dublin, Ireland). Department of Genetics, 2009)IncHI plasmids are prevalent amongst the Enterobacteriaceae and can confer a multi-drug resistance phenotype upon the host. IncHI plasmids are also thermosensitive for conjugative transfer. Conjugation can occur optimally ... -
Exploration of RNAi-based theraupeutic strategies for Dominant Dystrophic Epidermolysis Bullosa
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)The main focus of this Ph.D. thesis centres on exploration of a potential gene therapy for treatment of the dystrophic form of the rare blistering skin disorder Epidermolysis Bullosa (EB). There are three main subtypes of ... -
Functional analysis of a novel transmembrane protein, P1xdc2, in the developing nervous system of the chick and mouse
(Trinity College (Dublin, Ireland). Department of Genetics, 2009)Studies utilising the PLAP secretory trap method isolated a gene trap insertion in a novel gene (mouse line KST37) (Leighton et al., 2001) which has since been named Plexin domain containing 2 (Plxdc2). Plxdc2 encodes a ... -
The interaction of clumping factor B and iron regulated surface determinants of Staphylococcus aureus with platelets
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2009)Clumping factor B (ClfB) of Staphylococcus aureus is a multi-functional surface protein which binds to cytokeratin 10 and fibrinogen. Cytokeratin 10 is found on the surface of desquamated epithelial cells of the anterior ... -
Patterns of regulation of gene expression and genetic novelty in mammalian species
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)Genetic novelty is the driving force behind evolution. Consequently, a large amount of genetic research is dedicated to determining the mechanisms behind, and the effects of, the creation of new genes. Where and when these ... -
From single nucleotide polymorphisms to high-throughput sequencing in the complex genetics of amyotrophic lateral sclerosis
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2012)Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive weakening of limb and bulbar muscles resulting in paralysis and death from respiratory failure within three to five years ... -
Over-expression of SNAP-25 in neuronal tissues
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2012)The work described within this PhD thesis represents many years work on several different projects. In brief, SNAP-25 was identified as a downregulated candidate gene of interest from a comparative microarray study in the ... -
The identification and functional characterisation of caspase substrates involved in inflammation
(Trinity College (Dublin, Ireland). Department of Genetics, 2010)During inflammation the immune system responds to harmful stimuli, tries to control the stimuli and initiate a healing process for damaged tissue. After recognition of microbes by the innate immune system caspases are ... -
Investigating the roles of transmembrane semaphorins on thalamocortical connectivity
(Trinity College (Dublin, Ireland). Department of Genetics, 2009)The functional properties of structures in the central nervous system are critically dependent on the precision of neuronal connectivity. The cerebral cortex receives most of its input from the environment through ... -
Olfactory-avoidance habituation in Drosophila melanogaster
(Trinity College (Dublin, Ireland). Department of Genetics, 2015)Habituation is a form of sensory filtering in response to prolonged or repeated stimuli in the environment [Harris, 1943; Thompson and Spencer, 1966; Christoffersen, 1997; Rankin et al., 2009], It provides biological ... -
Detection of selection in mammalian genomes and populations
(Trinity College (Dublin, Ireland). Department of Genetics, 2010)Genome-wide scans for evidence of positive selection in mammalian genomes have recently become possible with the availability of whole genome sequences. They offer a chance to identify those genes that were of most importance ... -
Investigating mechanisms underlying olfactory habituation in Drosophila melanogaster
(Trinity College (Dublin, Ireland). Department of Genetics, 2010)Habituation is a common form of learning and memory that has been poorly studied despite its fundamental importance and clinical significance. During habituation, the behavioural response to a prolonged or repeated ... -
Optimisation of a suppression and replacement therapeutic strategy for animal models of rhodopsin-linked autosomal dominant Retinitis Pigmentosa
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2012)The focus of research presented in this PhD thesis was optimising a therapeutic strategy for an inherited retinal disorder termed Retinitis Pigmentosa (RP). RP is a disease in which the photoreceptors progressively degenerate. ... -
Rational design of artificial genetic switches : co-option of H-NS-repressed operons by the VirB virulence master regulator
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2012)The H-NS protein represses the transcription of hundreds of genes in Gram-negative bacteria. De-repression is achieved by a multitude of mechanisms, many of which involve binding of a protein to DNA at the repressed promoter ... -
Understanding the molecular interplay between bacterial pathogens and hosts : an evolutionary approach
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2012)The emergence of ecological adaptations is a fundamental conundrum in evolutionary biology. A magnificent and astonishing diversity of life forms occupy a myriad of ecological niches, from stable intra-cellular nutrition-rich ... -
Identification and characterisation of a novel Polycomb Repressive Complex 2 associated protein that is an alternatively spliced product of the LCOR gene locus
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2015)Polycombs are evolutionary conserved epigenetic regulators crucial for specification of cell types during development. They assemble in multiprotein complexes to modify amino terminal tails of histone H3 at lysine 27 to ... -
An investigation of the specificity of interaction in two-component systems using YycFG and PhoPR of Bacillus subtilis as a model system
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2009)Presented in this thesis is a study of the specificity of interaction between histidine kinases and response regulators in two-component systems using YycFG and PhoPR as model system in B. subtilis. -
Genomewide admixture mapping of African cattle
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)Genome-wide SNP analyses of cattle populations have become practical. In parallel, advanced and sophisticated statistical packages make it practical to extract embedded genetic information; for example differential levels ... -
Staphylococcus lugdunensis : Genome sequence, genetic systems, virulence and an amplifiable isd locus
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)Staphylococcus lugdunensis is a coagulase negative staphylococcus (CoNS). Infections caused by CoNS are normally less severe than infections caused by S. aureus. However, S. lugdunensis is associated with a series of severe ... -
Sustained expression of fis, the gene coding for the Fis nucleoid-associated protein, during the stationary phase of growth in Salmonella enterica
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)The Factor for Inversion Stimulation (Fis) is a global regulator of virulence genes in Salmonella enterica serovar Typhimurium. Previously, the Fis protein was throught to be expressed only during the early exponential ...