School of Genetics & Microbiology: Recent submissions
Now showing items 481-500 of 911
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Analysis of the chloramphenicol resistance determinant of plasmid R26
(Trinity College (Dublin, Ireland). Department of Microbiology, 1985)The Inc P-1 plasmid, R2B carries an inducible chloramphenicol resistance determinant, cml. This specifies low level chloramphenicol resistance (30 μg/ml] in Escherichia coli. The mechanism does not involve drug detoxification ... -
Efficient gene delivery to photoreceptors using AAV2/rh10 and rescue of the Rho�^'/�^' mouse
(2015)As gene therapies for various forms of retinal degeneration progress toward human clinical trial, it will be essential to have a repertoire of safe and efficient vectors for gene delivery to the target cells. Recombinant ... -
Targeting the NLRP3 inflammasome in chronic inflammatory diseases: Current perspectives
(2015)The inflammasome is a molecular platform formed by activation of an innate immune pattern recognition receptor seed, such as NLRP3. Once activated, NLRP3 recruits the adapter ASC (apoptosis-related speck-like protein ... -
Gene dosage in the dysbindin schizophrenia susceptibility network differentially affect synaptic function and plasticity
(2015)Neurodevelopmental disorders arise from single or multiple gene defects. However, the way multiple loci interact to modify phenotypic outcomes remains poorly understood. Here, we studied phenotypes associated with mutations ... -
The phenotypic plasticity of duplicated genes in Saccharomyces cerevisiae and the origin of adaptations
(2017)Gene and genome duplication are the major sources of biological innovations in plants and animals. Functional and transcriptional divergence between the copies after gene duplication has been considered the main driver of ... -
Essentiality is a strong determinant of protein rates of evolution during mutation accumulation experiments in Escherichia coli
(2016)The Neutral Theory of Molecular Evolution is considered the most powerful theory to understand the evolutionary behavior of proteins. One of the main predictions of this theory is that essential proteins should evolve ... -
The molecular chaperone dnak is a source of mutational robustness
(2016)Molecular chaperones, also known as heat-shock proteins, refold misfolded proteins and help other proteins reach their native conformation. Thanks to these abilities, some chaperones, such as the Hsp90 protein or the ... -
Recent positive selection has acted on genes encoding proteins with more interactions within the whole human interactome
(2015)Genes vary in their likelihood to undergo adaptive evolution. The genomic factors that determine adaptability, however, remain poorly understood. Genes function in the context of molecular networks, with some occupying ... -
A whole mitochondria analysis of the Tyrolean Iceman's leather provides insights into the animal sources of Copper Age clothing
(2016)The attire of the Tyrolean Iceman, a 5,300-year-old natural mummy from the Ötzal Italian Alps, provides a surviving example of ancient manufacturing technologies. Research into his garments has however, been limited by ... -
A genome-wide association study for genetic susceptibility to Mycobacterium bovis infection in dairy cattle identifies a susceptibility QTL on chromosome 23
(2016)Background Bovine tuberculosis (bTB) infection in cattle is a significant economic concern in many countries, with annual costs to the UK and Irish governments of approximately €190 million and €63 million, respectively, ... -
Genomic signals of migration and continuity in Britain before the Anglo-Saxons
(2016)The purported migrations that have formed the peoples of Britain have been the focus of generations of scholarly controversy. However, this has not benefited from direct analyses of ancient genomes. Here we report nine ... -
The N-end rule pathway regulates pathogen responses in plants.
(2016)To efficiently counteract pathogens, plants rely on a complex set of immune responses that are tightly regulated to allow the timely activation, appropriate duration and adequate amplitude of defense programs. The coordination ... -
Investigating the transcriptional regulation of small RNA expression in Salmonella enterica serovar Typhimurium
(Trinity College (Dublin, Ireland). Department of Microbiology, 2015)Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important foodborne pathogen that causes self-limiting gastroenteritis, or more serious systemic infections in susceptible hosts. S. Typhimurium can infect a ... -
The role of Bcl-2 family in mitochondrial homeostasis
(Trinity College (Dublin, Ireland). Department of Genetics, 2015)Mitochondria are required by cells to generate ATP in order to meet their energy requirements. The electron transport chain, within the mitochondria, pumps protons into the intermembrane space generating an electrochemical ... -
Genetic diagnosis and treatment of inherited retinopathies
(Trinity College (Dublin, Ireland). Department of Genetics, 2014)The first goal of this PhD was to improve and optimize a gene therapy based on the yeast protein Ndi 1 for Leber's Hereditary Optic Neuropathy (LHON), a mitochondrially-inherited retinal disorder. LHON is an incompletely ... -
The role of Polycomb Repressive Complex 2 associated proteins in mediating complex recruitment
(Trinity College (Dublin, Ireland). Department of Genetics, 2013)All multi-cellular organisms are constructed from a myriad of distinct cell types, each performing specialized functions. With very few exceptions all cell types contain the same genetic information encoded in the genome. ... -
Examination of transcriptional regulation and functional roles of axon guidance receptors in Drosophila dorsal vessel formation
(Trinity College (Dublin, Ireland). Department of Genetics, 2014)Early steps of cardiogenesis in vertebrates and invertebrates are similar in that in both systems, bilateral rows of cardiac progenitors migrate towards a point where the two rows meet and make a tubular structure. As with ... -
Enhancement of outflow facility in the murine eye by targeting selected tight-junctions of Schlemm's canal endothelia
(2017)The juxtacanalicular connective tissue of the trabecular meshwork together with inner wall endothelium of Schlemm’s canal (SC) provide the bulk of resistance to aqueous outflow from the anterior chamber. Endothelial cells ... -
Panel-Based population next-generation sequencing for inherited retinal degenerations
(2016)Inherited retinopathies affect approximately two and a half million people globally, yet the majority of affected patients lack clear genetic diagnoses given the diverse range of genes and mutations implicated in these ... -
The identification of novel peptides using phage display with the potential for use in breast cancer therapy
(Trinity College (Dublin, Ireland). Department of Microbiology, 2011)Breast cancer is a leading cause of cancer-related mortality in women. Current treatments for breast cancer include surgery, radiotherapy, chemotherapy and hormone therapy. The recent development of Herceptin has added a ...
