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dc.contributor.authorKENNY, ELAINEen
dc.contributor.authorMORRIS, DEREKen
dc.contributor.authorCORMICAN, PAULen
dc.date.accessioned2012-01-05T12:25:37Z
dc.date.available2012-01-05T12:25:37Z
dc.date.issued2011en
dc.date.submitted2011en
dc.identifier.citationFitzpatrick DA, O'Brien J, Moran C, Hasin N, Kenny E, Cormican P, Gates A, Morris DW, Jones GW, Assessment of Inactivating Stop Codon Mutations in Forty Saccharomyces cerevisiae Strains: Implications for [PSI] Prion- Mediated Phenotypes., PLoS One, 6, 12, e28684, 2011en
dc.identifier.issn1932-6203en
dc.identifier.otherYen
dc.descriptionPUBLISHEDen
dc.description.abstractThe yeast prion [PSI+] has been implicated in the generation of novel phenotypes by a mechanism involving a reduction in translation fidelity causing readthrough of naturally occurring stop codons. Some [PSI+] associated phenotypes may also be generated due to readthrough of inactivating stop codon mutations (ISCMs). Using next generation sequencing we have sequenced the genomes of two Saccharomyces cerevisiae strains that are commonly used for the study of the yeast [PSI+] prion. We have identified approximately 26,000 and 6,500 single nucleotide polymorphisms (SNPs) in strains 74-D694 and G600 respectively, compared to reference strain S288C. In addition to SNPs that produce non-synonymous amino acid changes we have also identified a number of SNPs that cause potential ISCMs in these strains, one of which we show is associated with a [PSI+]-dependent stress resistance phenotype in strain G600. We identified twenty-two potential ISCMs in strain 74-D694, present in genes involved in a variety of cellular processes including nitrogen metabolism, signal transduction and oxidative stress response. The presence of ISCMs in a subset of these genes provides possible explanations for previously identified [PSI+]-associated phenotypes in this strain. A comparison of ISCMs in strains G600 and 74-D694 with S. cerevisiae strains sequenced as part of the Saccharomyces Genome Resequencing Project (SGRP) shows much variation in the generation of strain-specific ISCMs and suggests this process is possible under complex genetic control. Additionally we have identified a major difference in the abilities of strains G600 and 74-D694 to grow at elevated temperatures. However, this difference appears unrelated to novel SNPs identified in strain 74-D694 present in proteins involved in the heat shock response, but may be attributed to other SNP differences in genes previously identified as playing a role in high temperature growth.en
dc.description.sponsorshipThis work was supported by a Science Foundation Ireland (www.sfi.ie) Research Frontiers grant (08/BMT/1439) awarded to GWJ. DAF acknowledges support of the Irish Health Research Board (www.hrb.ie). CM was supported by an IRCSET (www.ircset.ie) postgraduate scholarship and NH is supported by a NUI Maynooth John and Pat Hume postgraduate scholarship. The Trinity Genome Sequencing Laboratory is supported by Science Foundation Ireland.en
dc.language.isoenen
dc.relation.ispartofseriesPLoS Oneen
dc.relation.ispartofseries6en
dc.relation.ispartofseries12, e28684en
dc.rightsYen
dc.subjectGeneticsen
dc.subjectPSI+en
dc.titleAssessment of Inactivating Stop Codon Mutations in Forty Saccharomyces cerevisiae Strains: Implications for [PSI] Prion- Mediated Phenotypes.en
dc.typeJournal Articleen
dc.type.supercollectionscholarly_publicationsen
dc.type.supercollectionrefereed_publicationsen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/kennyelen
dc.identifier.peoplefinderurlhttp://people.tcd.ie/morrisdwen
dc.identifier.rssinternalid76541en
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0028684en
dc.subject.TCDThemeGenes & Societyen
dc.identifier.rssurihttp://dx.doi.org/10.1371/journal.pone.0028684en
dc.contributor.sponsorScience Foundation Ireland (SFI)en
dc.contributor.sponsorGrantNumber08/BMT/1439en
dc.contributor.sponsorIrish Research Council for Science and Engineering Technology (IRCSET)en
dc.contributor.sponsorHealth Research Board (HRB)en
dc.identifier.urihttp://hdl.handle.net/2262/61496


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