Biochemistry (Theses and Dissertations): Recent submissions
Now showing items 201-220 of 333
-
Analysis of the MAPK pathways, in silico and in vitro
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2002)This thesis takes a combined computational and experimental approach to study the MAPK pathways. These proteins were chosen as they are highly conserved in both sequence and function across all eukaryotes. An evolutionary ... -
Local cellular and humoral immunity to Bordetella pertussis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)The development of whole cell vaccine (Pw) and acellular pertussis vaccines (Pa) has had a huge impact on the incidence of whooping cough worldwide. The traditional Pw had high protective efficacy, but were associated with ... -
Modulation of the inflammatory response by taurine and its structural analogue, HEPES
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2000)Activated neutrophils (PMNs) have been implicated in the production of tissue injury in inflammatory-bowel disease (IBD) through release of proinflammatory mediaters during respiratory burst activity. Taurine, the most ... -
Studies on muscle residues of the anti-bacterial veterinary medicines tilmicosin, enrofloxacin and tiamulin in livestock and the risk to consumers from ingestion of injection-sites
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2001)Studies on incurred residues of the veterinary anti-bacterials tilmicosin, enrofloxacin and tiamulin were undertaken in cattle, sheep and pigs in order to investigate the effects of cooking on injection-site residues and ... -
NMR Spectroscopic Characterization of Proteins Large and Small: Aggregates, Oligomers, and Peptides
(Trinity College Dublin. School of Biochemistry & Immunology. Discipline of Biochemistry, 2019)Nuclear magnetic resonance (NMR) spectroscopy is one of the most powerful techniques for the investigation of structural, dynamic properties and interactions of biomolecules at the atomic level. In this thesis, by using ... -
Studies into the effects of the vaccinia virus protein A46R on Interleukin-1/Toll-Like receptor signalling pathways
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Poxviruses circumvent the host immune response by encoding proteins that can antagonise host defence mechanisms. The vaccinia virus (VV) protein, A46R, was identified based on sequence similarity to the Toll/Interleukin- ... -
A study of the effects of neurotoxins on the activity and copper of Ceruloplasmin
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)This thesis focuses on the ability of neurotoxins to affect the activity of the copper transport protein ceruloplasmin (EC 1.16.3.1; ferroxidase) and its intrinsic oxidase activities. The protective properties of taurine ... -
Modulation of Interleukin-1 and Toll-like receptor signalling by the Vaccinia virus protein A52R
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)Vaccinia virus (VV) has many m echanisms to suppress and modulate the host im mune response. Given the role of Toll-like receptors (TLRs) and interleukin 1 (IL-1) in immunity, it was possible that VV would have mechanisms ... -
Studies on the role of toll-like receptors in natural killer cell function
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)Natural Killer (NK) cells express receptors that allow them to recognise pathogen and activate effector functions such as cytotoxicity and cytokine production. Among these receptors are the recently identified Toll-like ... -
Studies into the phosphorylation of MyD88 adapter-like (Mal)
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)Members of the Toll-like receptor (TLR) family are essential players in activating the host innate immune response against infectious microorganisms. All TLRs signal through Toll/IL-1 receptor (TIR) domain-containing adapter ... -
Characterisation of signalling pathways activated by the IL-1 receptor homologue T1/ST2
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2003)T1/ST2 is a member of the IL-1 receptor family possessing the three characteristic extracellular Ig domains and an intracellular Toll-IL1R (TIR) domain. It is an orphan receptor expressed on a variety of cell types including ... -
Detection, expression and elucidation of the mechanism of action of mitochondrial uncoupling Protein 1
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)A system for overexpression of recombinant proteins in E. coli is defined. Using subcloning techniques the cDNAs for human uncoupling proteins (hUCP) 1, 2 and 3 were introduced to the pET expression system for E. coli. ... -
The role of IL-1 in the induction of IL-17 producing T cells
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2008)T helper cell responses have traditionally been divided into two distinct subsets, Th1 and Th2, producing IFN-y and IL-4 respectively. However, more recently the IL-12 family member, IL-23, has been shown to have a role ... -
Identification and characterisation of proteins from the tomato lectin binding fraction of Trypanosoma brucei
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2007)African trypanosomes, of which Trypanosoma brucei may be considered a prototypic member, are protozoan parasites responsible for sleeping sickness in man and related diseases in cattle and other domestic animals. Although ... -
Modulation of cellular immunity by Schistosoma mansoni in mice
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)Schistosoma mansoni is a trematode parasite that elicits a potent Th2 response in its mammalian host. Schistosomes are powerful stimulators and suppressors of the immune system, with these alterations in immune function ... -
Immunity and immune evasion in Hepatitis C virus infection
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2008)Hepatitis C virus (HCV) is an RNA virus which chronically infects an estimated 200 million people worldwide. Infection with HCV proceeds to chronicity in 80% of cases, and can be associated with the development of liver ... -
Modulation of innate and adaptive immune responses by adenylate cyclase toxin from Bordetella pertussis
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2006)CyaA belongs to the repeat-in-toxin (RTX) family of pore-forming toxins that require posttranslational acyiation to lyse eukaryotic cells. While it was known that CyaA binds to a variety of cell types, catalyses the ... -
Immediate-Early-Gene (IEG) expression in excitotoxicity
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2004)The mouse cerebellar granule cell system (mCGCs; Schousboe et al, 1989) was established in this laboratory (Bayer AG, Germany) as a suitable cell culture model for excitotoxicity studies. Suitability was confirmed by ... -
A study of the multiple functions and interactions of semicarbazide sensitive amine oxidase
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2005)This thesis focuses on three of the recognised multiple functions of mammalian semicarbazide sensitive amine oxid ase (SSAO) and the contribution of monoamine oxidase (MAO) to some of these. SSAO is a vascular-adhesion ... -
Functional consequences of genetic polymorphism of the KIR3DL1/S1 receptor
(Trinity College (Dublin, Ireland). School of Biochemistry and Immunology, 2007)Natural Killer (NK) cells are crucial effector cells of the innate immune system that function to eliminate virally infected and transformed cells. The activation of these cells is controlled in part by the expression of ...
