Browsing Microbiology (Theses and Dissertations) by Date of Publication
Now showing items 61-80 of 87
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Investigating the transcriptional regulation of small RNA expression in Salmonella enterica serovar Typhimurium
(Trinity College (Dublin, Ireland). Department of Microbiology, 2015)Salmonella enterica serovar Typhimurium (S. Typhimurium) is an important foodborne pathogen that causes self-limiting gastroenteritis, or more serious systemic infections in susceptible hosts. S. Typhimurium can infect a ... -
Investigating transcription and the histone methylation landscape in quiescent cells of Saccharomyces cerevisiae
(Trinity College (Dublin, Ireland). Department of Microbiology, 2015)Eukaryotic genomes are organised as the DNA-protein complex, chromatin within the cell nucleus. The fundamental subunit of chromatin is the nucleosome, which comprises 146 base pairs of DNA wrapped around an octamer of ... -
High-throughput investigation of virulence gene regulation by sRNAs in Salmonella Typhimurium
(Trinity College (Dublin, Ireland). Department of Microbiology, 2015)This study focused on novel gene regulatory systems in Salmonella enterics serovar Typhimurium, a model gram-negative, bacterial pathogen. The sRNA-mediated control of virulence genes was a particular emphasis. The research ... -
Preventing protein-dependent biofilm formation in Staphylococcus aureus by targeting the serine aspartate repeat protein C and fibronectin binding proteins
(Trinity College (Dublin, Ireland). Department of Microbiology, 2017)Staphylococcus aureus is a leading cause of biofilm infections on indwelling medical devices. S. aureus biofilm infections are intrinsically difficult to treat. They are recalcitrant to conventional antibiotics and resistant ... -
Examining the role of the probiotic candidate Lactobacillus salivarius in intestinal and immune homeostasis
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)A complex interplay exists between commensals of the gut microbiota, pathogenic bacteria and the host organism within the intestinal environment. The homeostasis of the intestinal epithelium and resident immune cells is ... -
Investigating factors that contribute to the ability of Staphylococcus aureus to colonise atopic dermatitis skin
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)The skin of atopic dermatitis (AD) patients is frequently colonised by Staphylococcus aureus. Colonisation by S. aureus correlates with disease severity and exacerbated disease symptoms. The factors involved in S. aureus ... -
FNR is a regulator of Salmonella pathogenicity Island 2 in Salmonella Typhimurium
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)During infection, S. Typhimurium employs Salmonella pathogenicity island (SPI)-encoded type three secretion systems (T3SS) 1 and 2 to invade and survive in host cells. However, expression of SPI-2 is seen at the epithelial ... -
Investigating influenza virus A pleomorphy
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)Clinical isolates of influenza A virus particles display a pleomorphic phenotype, where particles can range in size from 100 nm to 10 ?m in length while maintaining uniform diameters. Conversely, most laboratory-adapted ... -
Discovery and engineering of antimicrobial peptides to combat food spoilage
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)This work sought to use a bioinformatics based approach to discover plant derived antimicrobial peptides which were structurally similar to human β-defensin 3 (HBD3) and which possessed effective antimicrobial activity ... -
Consequences of exchanging the NAP-encoding genes fis and dps in Salmonella enterica Serovar Typhimurium
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2020)The genes encoding nucleoid associated proteins (NAPs) are syntenic among γ-Proteobacteria, and several factors are known to impact gene expression at a given locus, including changes in gene dosage caused by DNA replication. ... -
Unravelling a food derived bioactive peptide with dual functionality of antimicrobial and immunomodulatory properties
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2020)In this thesis, a computational data mining and feature based approach to unravel the abundance of unexplored peptides from nature and to identify potent, novel antimicrobial peptides (AMPs) from phyto-proteins is proposed. ... -
Investigating chromatin remodelling by the Swi-Snf and Tup1-Cyc8 complexes
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2020)Swi-Snf is an ATP-dependent chromatin remodelling complex which generally acts as a co-activator of gene transcription via its removal of promoter nucleosomes. Conversely, Tup1-Cyc8 (Ssn6) is a co-repressor complex which ... -
Investigating the interplay between the Tup1-Cyc8 and Swi-Snf chromatin remodelling complexes in Saccharomyces cerevisiae
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)Swi-Snf is an ATP dependent chromatin remodelling complex which acts as a co-activator of gene transcription by its ability to open up densely packed chromatin by removal of nucleosomes. Conversely, Tup1-Cyc8 ... -
Drug Repurposing as a Strategy to Uncover Effective Antibacterial Compounds using Salmonella as a model
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)Without doubt, antimicrobial resistance (AMR) is one of the most serious global challenge for public, animals and environmental health. Tackling this huge issue that is threatening modern medicine, is an urgent priority ... -
Impact of Metabolites on the Intestinal Mucosa and Development of Inflammation
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)The intestinal epithelial mucosal barrier plays a pivotal role in the maintenance of intestinal homeostasis, acting as an important barrier between microbes and the host s innate immune system. The metabolite-rich environment ... -
Significance of the chromosomal positions of the genes encoding Integration Host Factor and DNA gyrase in Salmonella.
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)In this thesis, two studies are described that built upon the idea of the chromosomal gene importance. In both studies Salmonella was used as a model organism. In the first study, ihfA and ihfB ? genes that encode ... -
Functional characterisation of small RNAs in Acinetobacter baumannii using Hi-GRIL-seq
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)Acinetobacter baumannii is a priority pathogen that is a leading source of nosocomial multidrug resistant (MDR) infections worldwide. A key to the success of A. baumannii is the ability to quickly adapt to changing ... -
Investigation of the cellular location of Multicopper Oxidase and its contribution to copper tolerance and immune resistance in Staphylococcus aureus
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2022)In high amounts copper exerts toxic effects on bacteria, and therefore its cellular concentrations must be tightly controlled. Staphylococcus aureus is a WHO priority pathogen responsible of an enormous burden on healthcare ... -
Accelerated Evolution of Lager Yeast Strains for Improved Flavour Profiles
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2022)S. pastorianus is an interspecific hybrid resulting from natural hybridization between S. cerevisiae and S. eubayanus. These two species belong to the Saccharomyces genus, a genus that encompasses different species related ... -
Understanding the Role of Host Factors in the Modulation of Virulence Factors of Staphylococcus aureus.
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023)Staphylococcus aureus is adept at colonising and infecting the human host. Host factors are known to influence pathogen physiology and this thesis aimed to examine the effects of human bile and human plasmin on S. aureus. ...