Microbiology: Recent submissions
Now showing items 61-80 of 333
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R factor tetracycline and chloramphenicol resistance in Escherichia coli K12 cmlB mutants
(1975)The isolation of Escherichia coli chromosomal mutants that increased the level of resistance of a partially tetracycline-sensitive mutant of RI00-I is described. Plasmid-less derivatives of these moderately resistant mutants ... -
Recombination at the coagulase locus in Staphylococcus aureus: plasmid integration and amplification
(1993)The integrating plasmid pCOA18, comprising pUC18 linked to a mutated coagulase (coa) gene from Staphylococcus aureus, and constructed by substituting coa sequences with a tetracycline (Tc)-resistance marker (delta coa::Tcr), ... -
The gamma-hemolysin locus of Staphylococcus aureus comprises three linked genes, two of which are identical to the genes for the F and S components of leukocidin
(1993)The Staphylococcus aureus gamma-hemolysin comprises two polypeptides, whereas the gamma-hemolysin locus (hlg) contains three open reading frames. The hlgA and hlgB genes encode the gamma 1 and gamma 2 components, respectively. ... -
Quantitative comparison of clumping factor- and coagulase-mediated Staphylococcus aureus adhesion to surface-bound fibrinogen under flow
(1995)The contributions of clumping factor and coagulase in mediating Staphylococcus aureus adhesion to surface-adsorbed fibrinogen have been quantified by using a new methodology and analysis. The attachment or detachment ... -
Tn5 insertion mutations in the mercuric ion resistance genes derived from plasmid R100
(1983)The mercuric resistance (mer) genes of plasmid R100 were cloned into plasmid pBR322. A series of transposon Tn5 insertion mutations in the mer genes were isolated and mapped. The mutants were characterized phenotypically ... -
Analysis of tetracycline resistance encoded by transposon Tn10: deletion mapping of tetracycline-sensitive point mutations and identification of two structural genes
(1983)Deletions in the tet genes derived from Tn10 were formed from different tet::Tn5 insertion mutations by removing DNA sequences located between a HindIII site in Tn5 and a HindIII site adjacent to the tet genes. ... -
Posttranscriptional regulation of the inducible nonenzymatic chloramphenicol resistance determinant of IncP plasmid R26
(1985)The inducible nonenzymatic chloramphenicol resistance (Cmr) determinant of the IncP plasmid R26 was cloned on a 1,900-base-pair restriction endonuclease HindIII fragment. Transposon Tn5 mutagenesis revealed that at least ... -
Identification of the merR gene of R100 by using mer-lac gene and operon fusions
(1985)Transcriptional (operon) and translational (gene) fusions between the R100 merR gene and lacZ were constructed in vitro in a pBR322 plasmid carrying the mer genes derived from plasmid R100. The translational fusions were ... -
Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia coli K12 chromosome
(1975)Pairs of normally incompatible derivatives of R100-1 (one ChlS TetR, the other ChilR TetS) were forced to coexist in a recA host by selection for ChlR TetR cells. After many generations stable derivatives were isolated. ... -
Deletions in the r-determinant mer region of plasmid R100-1 selected by loss of mercury hypersensitivity
(1979)A mutant of plasmid R100-1, which conferred cellular hypersensitivity to Hg2+ because of the insertion of Tn801 (TnA) into the gene determining synthesis of mercuric reductase enzyme, allowed further mutational events to ... -
Recombination and complementation between R factors in Escherichia coli K12
(1971)Recombination between chloramphenicol-sensitive (Cms) mutants of Rl, and R100, has been demonstrated in Escherichia coli K12 rec+; it occurs at reduced frequency in recB and recC, and is not detectable in reeA, indicating ... -
Deletion map of the chloramphenicol resistance region of R1 and R100-1
(1973)Recombination between single-site and multisite chloramphenicol-sensitive mutants of the F-like R factors R1 and R100-1 indicates that the chloramphenicol resistance region is a single structural gene coding for the ... -
Fibronectin Binding Proteins SpsD and SpsL Both Support Invasion of Canine Epithelial Cells by Staphylococcus pseudintermedius
(2015)In this study, we investigated the cell wall-anchored fibronectin-binding proteins SpsD and SpsL from the canine commensal and pathogen Staphylococcus pseudintermedius for their role in promoting bacterial invasion of ... -
Transforming the untransformable:application of direct transformation to manipulate genetically Staphylococcus aureus and Staphylococcus epidermidis
(2012)The strong restriction barrier present in Staphylococcus aureus and Staphylococcus epidermidis has limited functional genomic analysis to a small subset of strains that are amenable to genetic manipulation. Recently, a ... -
Role for the A domain of unprocessed accumulation-associated protein (Aap) in the attachment phase of the Staphylococcus epidermidis biofilm phenotype
(2014)The polysaccharide intercellular adhesin or the cell wall-anchored accumulation-associated protein (Aap) mediates cellular accumulation during Staphylococcus epidermidis biofilm maturation. Mutation of sortase, which anchors ... -
Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus
(2014)Staphylococcus aureus is an important opportunistic pathogen and persistently colonizes about 20% of the human population. Its surface is 'decorated' with proteins that are covalently anchored to the cell wall peptidoglycan. ... -
Fibrinogen and elastin bind to the same region within the A domain of fibronectin binding protein A, an MSCRAMM of Staphylococcus aureus
(2007)The fibronectin binding protein, FnBPA, is a multifunctional microbial surface component recognizing adhesive matrix molecule (MSCRAMM) that promotes bacterial adherence to immobilized fibrinogen and elastin via the ... -
Staphylococcus aureus protein A binding to von Willebrand factor A1 domain is mediated by conserved IgG binding regions
(2006)Protein A (Spa) is a surface-associated protein of Staphylococcus aureus best known for its ability to bind to the Fc region of IgG. Spa also binds strongly to the Fab region of the immunoglobulins bearing V(H)3 heavy ... -
Clumping factor A of Staphylococcus aureus inhibits phagocytosis by human polymorphonuclear leucocytes
(2006)Staphylococcus aureus is a major cause of nosocomial and community-acquired infection. It expresses several factors that promote avoidance of phagocytosis by polymorphonuclear leucocytes. Clumping factor A (ClfA) is a ... -
Fibronectin‐binding proteins of Staphylococcus aureus mediate activation of human platelets via fibrinogen and fibronectin bridges to integrin GPIIb/IIIa and IgG binding to the FcγRIIa receptor
(2006)Staphylococcus aureus is a leading cause of infective endocarditis (IE). Platelet activation promoted by S. aureus resulting in aggregation and thrombus formation is an important step in the pathogenesis of IE. Here, we ...
