Genetics: Recent submissions
Now showing items 141-160 of 578
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Next generation sequencing of ancient DNA
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)The application of next generation sequencing (NGS) technologies into the field of ancient DNA has seen a paradime shift in the level of analysis that can be completed using archaeological samples. However, to fully access ... -
Surface proteins and restriction-modification systems of bovine mastitis strains of Staphylococcus aureus
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)Staphylococcus aureus is frequently associated with bovine mastitis, one of the most economically important diseases in the dairy industry. The bovine S. aureus strain RF122 represents a geographically widespread clone ... -
Population genetics of modern and ancient cattle
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)The domestication of cattle was a milestone in human history. It is widely accepted that cattle were domesticated in at least two independent domestication events; the Fertile Crescent was the primary domestication centre ... -
Characterisation of the second binding protein of immunoglobulin (SBi) in Straphylococcus aureus
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)The second binding protein for immunoglobulin (Sbi) of Staphylococcus aureus comprises two IgG binding domains (D1 and D2) that share 30% sequence homology with the IgG binding domains of protein A (Spa) and two domains ... -
Investigation of the regulation of Bcl-2 family members in apoptosis and cancer
(Trinity College (Dublin, Ireland). Department of Genetics, 2010)Apoptosis is a highly controlled mechanism of cell suicide that is activated in response to cellular stress and damage. The Bcl-2 family of proteins play a key role in the regulation of this process. Opposing subsets of ... -
Specification of floral reproductive organs : the role of AGAMOUS
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)Homeosis is referred to as the transformation of an organ's identity toward the identity of another organ. This term was coined in 1894 and soon thereafter; these organ transformations were attributed to the variation in ... -
Analysis of the gene regulatory network underlying reproductive floral organ development through gene perturbation experiments
(Trinity College (Dublin, Ireland). Department of Genetics, 2013)Homeosis or homeotic transformation refers to the formation of one body structure or organ in place of another. For more than two decades, the floral homeotic genes have been at the center of intense studies that showed ... -
Defining the role of the AP2/ERF transcription factors Rap2.4 and Rap2.4b in stress responses in the model plant Arabidopsis thaliana
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2010)This study characterises the molecular properties and the function of two Arahidopsis AP2/ERF transcription factors, Rap2.4 and Rap2.4b. The two genes belong to the DREB subgroup, members of which have been demonstrated ... -
Evolution of the OmpR regulon in Salmonella enterica serovar Typhimurium and Escherichia coli in response to low pH
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2013)Two-component systems (TCS) enable bacteria to sense, respond and adapt to a wide range of environmental stimuli. The OmpR/EnvZ TCS is composed of the inner membrane sensor kinase EnvZ and the response regulator OmpR. In ... -
Automated annotation of yeast genomes
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)I have built an automated annotation pipeline (named YGAP - Yeast Genome Annotation Pipeline), designed specifically for new yeast genome sequences lacking transcriptome data. YGAP uses homology and synteny information ... -
Picking the high hanging fruit : automated ways to annotate awkward genes
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)In Chapter 2 I describe the development of software called SearchDOGS (Database of Orthologous Genomic Segments). By identifying regions of conserved local synteny across species using the synteny information contained in ... -
Control of conjugation in the plasmid pSf-R27
(Trinity College (Dublin, Ireland). Department of Genetics, 2009)IncHI plasmids are prevalent amongst the Enterobacteriaceae and can confer a multi-drug resistance phenotype upon the host. IncHI plasmids are also thermosensitive for conjugative transfer. Conjugation can occur optimally ... -
Exploration of RNAi-based theraupeutic strategies for Dominant Dystrophic Epidermolysis Bullosa
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2011)The main focus of this Ph.D. thesis centres on exploration of a potential gene therapy for treatment of the dystrophic form of the rare blistering skin disorder Epidermolysis Bullosa (EB). There are three main subtypes of ... -
Functional analysis of a novel transmembrane protein, P1xdc2, in the developing nervous system of the chick and mouse
(Trinity College (Dublin, Ireland). Department of Genetics, 2009)Studies utilising the PLAP secretory trap method isolated a gene trap insertion in a novel gene (mouse line KST37) (Leighton et al., 2001) which has since been named Plexin domain containing 2 (Plxdc2). Plxdc2 encodes a ... -
The interaction of clumping factor B and iron regulated surface determinants of Staphylococcus aureus with platelets
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2009)Clumping factor B (ClfB) of Staphylococcus aureus is a multi-functional surface protein which binds to cytokeratin 10 and fibrinogen. Cytokeratin 10 is found on the surface of desquamated epithelial cells of the anterior ... -
Patterns of regulation of gene expression and genetic novelty in mammalian species
(Trinity College (Dublin, Ireland). Department of Genetics, 2012)Genetic novelty is the driving force behind evolution. Consequently, a large amount of genetic research is dedicated to determining the mechanisms behind, and the effects of, the creation of new genes. Where and when these ... -
From single nucleotide polymorphisms to high-throughput sequencing in the complex genetics of amyotrophic lateral sclerosis
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2012)Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by progressive weakening of limb and bulbar muscles resulting in paralysis and death from respiratory failure within three to five years ... -
Over-expression of SNAP-25 in neuronal tissues
(Trinity College (Dublin, Ireland). School of Genetics and Microbiology, 2012)The work described within this PhD thesis represents many years work on several different projects. In brief, SNAP-25 was identified as a downregulated candidate gene of interest from a comparative microarray study in the ... -
The identification and functional characterisation of caspase substrates involved in inflammation
(Trinity College (Dublin, Ireland). Department of Genetics, 2010)During inflammation the immune system responds to harmful stimuli, tries to control the stimuli and initiate a healing process for damaged tissue. After recognition of microbes by the innate immune system caspases are ... -
Investigating the roles of transmembrane semaphorins on thalamocortical connectivity
(Trinity College (Dublin, Ireland). Department of Genetics, 2009)The functional properties of structures in the central nervous system are critically dependent on the precision of neuronal connectivity. The cerebral cortex receives most of its input from the environment through ...
