dc.contributor.advisor | Atkins, Gregory | |
dc.contributor.author | Gowda C. P. Chikkanna | |
dc.date.accessioned | 2019-04-30T08:48:24Z | |
dc.date.available | 2019-04-30T08:48:24Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Gowda C. P. Chikkanna, 'Tumour therapy using cytokine-expressing semliki forest virus vectors', [thesis], Trinity College (Dublin, Ireland). Department of Microbiology, 2005, pp 283 | |
dc.identifier.other | THESIS 7937 | |
dc.description.abstract | Semliki Forest Virus (SFV) vector is a transient RNA based suicidal
expression vector system and has been previously used as a potential anti-cancer
agent. Recently, a new enhanced SFV vector has been developed, pSFV10-E. Cells
transfected with this vector yield up to ten times more foreign protein than cells
transfected with original (non-enhanced) expression vector pSFV10. The two IL-12
gene subunits were cloned from mouse splenocytes and inserted into the pSFV10-E
and pSFV10 vectors. Both the pSFV-mIL-12 constructs were characterised for their
expression levels qualitatively and quantitatively in BHK-21 and K-BALB cells. The
secretion of biologically active mIL-12 was confirmed by inducing splenocytes for
IFN-y production using IL-12 expressed by BHK-21 and K-BALB cells. K-BALB,
CT26, and 4T1 tumours are the experimental tum our models in our study. | |
dc.format | 1 volume | |
dc.language.iso | en | |
dc.publisher | Trinity College (Dublin, Ireland). Department of Microbiology | |
dc.relation.isversionof | http://stella.catalogue.tcd.ie/iii/encore/record/C__Rb12731632 | |
dc.subject | Microbiology, Ph.D. | |
dc.subject | Ph.D. Trinity College Dublin | |
dc.title | Tumour therapy using cytokine-expressing semliki forest virus vectors | |
dc.type | thesis | |
dc.type.supercollection | thesis_dissertations | |
dc.type.supercollection | refereed_publications | |
dc.type.qualificationlevel | Doctoral | |
dc.type.qualificationname | Doctor of Philosophy (Ph.D.) | |
dc.rights.ecaccessrights | openAccess | |
dc.format.extentpagination | pp 283 | |
dc.description.note | TARA (Trinity's Access to Research Archive) has a robust takedown policy. Please contact us if you have any concerns: rssadmin@tcd.ie | |
dc.identifier.uri | http://hdl.handle.net/2262/86348 | |