Structure-function analysis of the Fim-B and Fim-E site-specific recombinases of Escherichia coli K-12
Citation:
Matthew Patrick McCusker, 'Structure-function analysis of the Fim-B and Fim-E site-specific recombinases of Escherichia coli K-12', [thesis], Trinity College (Dublin, Ireland). Department of Microbiology, 2006, pp 370Abstract:
Phase variable expression of type 1 fimbriae in Escherichia coli arises from a site-specific
recombination event that inverts a 314 bp segment of chromosomal DNA
(fimS) carrying the promoter for transcription of the gene encoding the fimbrial
subunit protein. Two integrase-like recombinases that are 48% identical are involved
in switching. The FimB recombinase inverts the switch in either orientation, whereas
the FimE protein inverts it predominantly in the ON-to-OFF direction. The FimB and
FimE recom binases recognise core-binding sequences at either end of fimS. Inversion
of fimS involves site-specific recom bination reaction between two 9 bp inverted
repeats that for part of the core binding sequences.
Author: McCusker, Matthew Patrick
Advisor:
Dorman, CharlesPublisher:
Trinity College (Dublin, Ireland). Department of MicrobiologyNote:
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Full text availableKeywords:
Microbiology, Ph.D., Ph.D. Trinity College DublinMetadata
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