dc.contributor.advisor | Fleming, Alastair | en |
dc.contributor.author | Byrne, Nicole | en |
dc.date.accessioned | 2021-08-19T08:04:21Z | |
dc.date.available | 2021-08-19T08:04:21Z | |
dc.date.issued | 2021 | en |
dc.date.submitted | 2021 | en |
dc.identifier.citation | Byrne, Nicole, Investigating the interplay between the Tup1-Cyc8 and Swi-Snf chromatin remodelling complexes in Saccharomyces cerevisiae, Trinity College Dublin.School of Genetics & Microbiology, 2021 | en |
dc.identifier.other | Y | en |
dc.description | APPROVED | en |
dc.description.abstract | Swi-Snf is an ATP dependent chromatin remodelling complex which acts as a co-activator of gene transcription by its ability to open up densely packed chromatin by removal of nucleosomes. Conversely, Tup1-Cyc8 is a co-repressor complex that has the ability to position nucleosomes at the promotors of genes, forming a more closed chromatin state which is repressive towards transcription. The antagonistic activity of these two complexes has recently been shown to regulate102 genes by RNA-Seq analysis. In this study I have validated the subset of genes suggested to be under the co-regulation of Swi-Snf and Tup1-Cyc8 using gene-specific RT-qPCR and ChIP analysis. Using ChIP analysis of Snf2 and Tup1 proteins at gene promotors, examples of some of the co-regulated genes wereshown to be under the direct control of Swi-Snf and Tup1-Cyc8. The data also revealed that at these co-regulated genes Tup1occupancywas dependent on Snf2. Furthermore, Tup1 can persist at co-regulated genes in the absence of Cyc8 and that this retention of Tup1 is Snf2dependent. Finally, the data revealed that there may be two models of co-regulation. In model one, Tup-Cyc8 occupies the promotor of target genes to bring about repression. In the absence of Tup1-Cyc8, Swi-Snf is recruited resulting in gene activation. In model two, Swi-Snf and Tup1-Cyc8 both occupy the promotors of target genes with Swi-Snf becoming further enriched upon target gene activation in the absence of Tup1-Cyc8. Moreover, preliminary data might suggest that the two complexes interact with one another via the Swi3 and Cyc8 proteins. | en |
dc.publisher | Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology | en |
dc.rights | Y | en |
dc.title | Investigating the interplay between the Tup1-Cyc8 and Swi-Snf chromatin remodelling complexes in Saccharomyces cerevisiae | en |
dc.type | Thesis | en |
dc.type.supercollection | thesis_dissertations | en |
dc.type.supercollection | refereed_publications | en |
dc.type.qualificationlevel | Masters (Research) | en |
dc.identifier.peoplefinderurl | https://tcdlocalportal.tcd.ie/pls/EnterApex/f?p=800:71:0::::P71_USERNAME:BYRNEN24 | en |
dc.identifier.rssinternalid | 232656 | en |
dc.rights.ecaccessrights | openAccess | |
dc.identifier.uri | http://hdl.handle.net/2262/96845 | |