Browsing Microbiology (Theses and Dissertations) by Title
Now showing items 17-36 of 87
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Characterization of peptide mimics to antigens chaperoned by Hsp70 in MDA-MB-231 breast tumour cells
(Trinity College (Dublin, Ireland). Department of Microbiology, 2005)Tumour-derived heat shock protein (Hsp) preparations have been shown to elicit anti-tumour immune responses in both mice and man. Analysis of Hsp preparations from tumour cells revealed that the immuno-protective property ... -
Consequences of exchanging the NAP-encoding genes fis and dps in Salmonella enterica Serovar Typhimurium
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2020)The genes encoding nucleoid associated proteins (NAPs) are syntenic among γ-Proteobacteria, and several factors are known to impact gene expression at a given locus, including changes in gene dosage caused by DNA replication. ... -
Construction and characterisation of a Semliki Forest virus vector based rubella prototype vaccine
(Trinity College (Dublin, Ireland). Department of Microbiology, 2007)Alphavirus vaccines such as those based on the Semliki Forest virus (SFV) replicon have been widely studied as candidate vaccines. Following intramuscular injection, dispersal of the RNA occurs to the local lymph node only, ... -
Construction and development of simliki forest virus vectors for transient gene therapy of model tumours in mice
(Trinity College (Dublin, Ireland). Department of Microbiology, 2010)Cancer is a molecular and genetic disease that results from multiple alterations of genes, including proto-oncogenes and tumour-suppressor genes. Virotherapy is a branch of cancer gene therapy where oncolytic viruses are ... -
Development of SFV-based plasmid electroporation for use in tumour treatment and vaccine delivery
(Trinity College (Dublin, Ireland). Department of Microbiology, 2008)Electroporation is the delivery of short and intense electric pulses into living cells (either in tissue or suspension), which results in a transient and reversible increase in permeability of the cell transmembrane. This ... -
Discovery and engineering of antimicrobial peptides to combat food spoilage
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)This work sought to use a bioinformatics based approach to discover plant derived antimicrobial peptides which were structurally similar to human β-defensin 3 (HBD3) and which possessed effective antimicrobial activity ... -
Distinct phenotypes and transcriptomes of tup1 and cyc8 mutants suggest novel roles for TUP1 and CYC8 in Saccharomyces cerevisiae
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023)The Tup1-Cyc8 (Ssn6) co-repressor complex is a regulator of gene transcription in the yeast Saccharomyces cerevisiae. This project provides insights into the distinct roles of Tup1p and Cyc8p in gene repression, identifies ... -
Drug Repurposing as a Strategy to Uncover Effective Antibacterial Compounds using Salmonella as a model
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)Without doubt, antimicrobial resistance (AMR) is one of the most serious global challenge for public, animals and environmental health. Tackling this huge issue that is threatening modern medicine, is an urgent priority ... -
Effect of intranasal administration of Semliki Forest virus recombinant particles expressing interferon-beta on the progression of experimental autoimmune encephalomyelitis
(Trinity College (Dublin, Ireland). Department of Microbiology, 2006)Multiple sclerosis (MS) is an autoimmune demyelinating disease of the CNS, characterized by the presence of sclerotic lesions throughout the brain. MS is thought to be a CD4+ T helper 1 disease caused by the infiltration ... -
Epidemiology of Streptococcus agalactiae (Group B Streptococci) in Dublin Maternity Hospitals
(Trinity College (Dublin, Ireland). Department of Microbiology, 2002)Streptococcus agalactiae (Group B Streptococci) are Gram-positive encapsulated cocci distinguished from other streptococci by their narrower zones of P-haemolysis. Group B Streptococci (GBS) was first described as an ... -
Examining the role of the probiotic candidate Lactobacillus salivarius in intestinal and immune homeostasis
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)A complex interplay exists between commensals of the gut microbiota, pathogenic bacteria and the host organism within the intestinal environment. The homeostasis of the intestinal epithelium and resident immune cells is ... -
Fibronectin-binding protein B variation in straphylococcus aureus
(Trinity College (Dublin, Ireland). Department of Microbiology, 2010)The surface-expressed fibronectin-binding proteins FnBPA and FnBPB of Staphylococcus aureus mediate attachment to immobilised fibronectin, fibrinogen and elastin. These proteins are encoded by two closely linked but ... -
FNR is a regulator of Salmonella pathogenicity Island 2 in Salmonella Typhimurium
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)During infection, S. Typhimurium employs Salmonella pathogenicity island (SPI)-encoded type three secretion systems (T3SS) 1 and 2 to invade and survive in host cells. However, expression of SPI-2 is seen at the epithelial ... -
Functional characterisation of small RNAs in Acinetobacter baumannii using Hi-GRIL-seq
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)Acinetobacter baumannii is a priority pathogen that is a leading source of nosocomial multidrug resistant (MDR) infections worldwide. A key to the success of A. baumannii is the ability to quickly adapt to changing ... -
Gene regulation and the Fis nucleoid-associated protein in Salmonella enterica serovar Typhimurium
(Trinity College (Dublin, Ireland). Department of Microbiology, 2005)The Fis protein was found to influence the superhelical density of DNA and its ability to do this differed between Salmonella enterica serovar Typhimurium (S. typhimurium) and Escherichia coli (E. coli). Induction of the ... -
Gene regulation and the leucine-responsive regulatory protein of Salmonella enterica serovar Typhimurium
(Trinity College (Dublin, Ireland). Department of Microbiology, 2007)The leucine-responsive regulatory protein of Escherichia coli (E. coli) is a well characterised global regulator of transcription. Comparatively little is known about the Lrp regulon of Salmonella enterica serovar ... -
Genetic and Molecular Studies of Virulence Factors of Staphylococcus aureus
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023) -
Genetic diversity of Helicobactor pylori isolates through Microevolution in vivo
(Trinity College (Dublin, Ireland). Department of Microbiology, 2004)Chronic infection with H. pylori causes peptic ulcer disease and its presence is strongly linked with gastric adenocarcinoma and mucosa-associated lymphoid tissue (MALT) lymphoma. One of the most interesting characteristics ... -
Helicobacter pylori infection : in vitro diagnostic methods, antimicrobial susceptibility testing, epidemiology of recrudescence and clarithromycin resistance
(Trinity College (Dublin, Ireland). Department of Microbiology, 2000)H. pylori is a Gram-negative, non-spore forming, curved bacterium that colonises the gastric epithelium of the human stomach. H. pylori is firmly established as an aetiological agent in peptic ulcer disease and successful ... -
High-throughput investigation of virulence gene regulation by sRNAs in Salmonella Typhimurium
(Trinity College (Dublin, Ireland). Department of Microbiology, 2015)This study focused on novel gene regulatory systems in Salmonella enterics serovar Typhimurium, a model gram-negative, bacterial pathogen. The sRNA-mediated control of virulence genes was a particular emphasis. The research ...