Browsing Microbiology (Theses and Dissertations) by Title
Now showing items 6-25 of 87
-
Analysis of the genomic organisation and gene expression of brewery strains of yeast
(Trinity College (Dublin, Ireland). Department of Microbiology, 2008)The genomes of lager yeast arose from the fusion of two yeast strains most closely resembling Saccharomyces cerevisiae and Saccharomyces bayanus. The resultant strains have subsequently undergone genome duplications ... -
Analysis of the role of human papillomaviruses and the p53/Rb tumour suppressor genes in the development of cutaneous squamous cell carcinoma
(Trinity College (Dublin, Ireland). Department of Microbiology, 2000)Nonmelanoma skin cancers (NMSC) are the most frequent cancers in Caucasian populations. Renal transplant recipients (RTR) are at an increased risk of neoplasia, particularly skin cancer. Studies on anogenital cancer have ... -
Antimicrobial peptide preventing beer spoilage
(Trinity College (Dublin, Ireland). Department of Microbiology, 2012)Beer spoilage micro-organisms (BSMs) are a common threat to Master Brewers worldwide. Numerous studies have been carried out to date to overcome this problem, yet the most common preventative measure is the addition of ... -
Biasing switching outcomes in the Escherichia coli fim site-specific recombination system through DNA supercoiling and nucleoid-associated proteins
(Trinity College (Dublin, Ireland). Department of Microbiology, 2010)Type 1 fimbriae are typically expressed in nutrient poor environments and facilitate colonization through attachment when the bacterium can no longer support a motile lifestyle that demands a high metabolic flux. They play ... -
Biogenesis of histone mRNAs in the yeast Saccharomyces cerevisiae
(Trinity College (Dublin, Ireland). Department of Microbiology, 2007)The typical eukaryotic human diploid cell contains 3.2x10 9 base pairs of deoxyribonucleic acid (DNA) which, if presented in an extended form, would measure 1.2m in length. The large amount of DNA is tightly wrapped and ... -
Biomass to biofuel : the engineering of Saccharomyces species for the co-fermentation of cellulose and xylose
(Trinity College (Dublin, Ireland). Department of Microbiology, 2015)Lignocellulose represents one of the most abundant biomass sources in the world. Its renewable and abundant nature makes it a prime target for use in bioethanol production. The efficient conversion of lignocellulosic biomass ... -
Biomass to biofuel : towards the bioengineering of Saccharomyces species for cellulose degradation
(Trinity College (Dublin, Ireland). Department of Microbiology, 2011)Cellulose is the most abundant polysaccharide on earth and therefore represents a major reservoir of sugar that could be potentially converted to alcohol and used as a fuel source. The filamentous fungus Trichoderma reesei ... -
Characterisation of genotypic and phenotypic differences between Candida dubliniensis and Candida albicans
(Trinity College (Dublin, Ireland). Department of Microbiology, 2001)Summary -Candida dubliniensis is a recently described Candida species associated with oral candidosis in Human Immunodeficiency Virus (HIV)-infected and AIDS patients. The phylogenetic position of C. dubliniensis has ... -
Characterisation of SdrC, SdrD and SdrE from the serine-aspartate repeat family of Staphylococcus auerus
(Trinity College (Dublin, Ireland). Department of Microbiology, 2001)Staphylococcus aureus is an important human pathogen. The ability of S. aureus to initiate infection is attributed at least partly to the expression of cell wall-associated proteins known as MSCRAMMs (microbial surface ... -
Characterisation of Sfh, a novel H-NS-like protein
(Trinity College (Dublin, Ireland). Department of Microbiology, 2006)Members of the H-NS protein family are widespread among Gram-negative bacteria and are known to influence gene expression at a global level. Shigella flexneri serotype 2a strain 2457T expresses three members of the H-NS ... -
Characterisation of the Rns transcriptional regulator of enterotoxigenic Escherichia coli and regulationf of CS1 fimbrial expression
(Trinity College (Dublin, Ireland). Department of Microbiology, 2008)Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea amongst children in developing countries and travellers to such countries. Virulence in ETEC is dependent on both toxin production and adherence of the ... -
Characterization of peptide mimics to antigens chaperoned by Hsp70 in MDA-MB-231 breast tumour cells
(Trinity College (Dublin, Ireland). Department of Microbiology, 2005)Tumour-derived heat shock protein (Hsp) preparations have been shown to elicit anti-tumour immune responses in both mice and man. Analysis of Hsp preparations from tumour cells revealed that the immuno-protective property ... -
Consequences of exchanging the NAP-encoding genes fis and dps in Salmonella enterica Serovar Typhimurium
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2020)The genes encoding nucleoid associated proteins (NAPs) are syntenic among γ-Proteobacteria, and several factors are known to impact gene expression at a given locus, including changes in gene dosage caused by DNA replication. ... -
Construction and characterisation of a Semliki Forest virus vector based rubella prototype vaccine
(Trinity College (Dublin, Ireland). Department of Microbiology, 2007)Alphavirus vaccines such as those based on the Semliki Forest virus (SFV) replicon have been widely studied as candidate vaccines. Following intramuscular injection, dispersal of the RNA occurs to the local lymph node only, ... -
Construction and development of simliki forest virus vectors for transient gene therapy of model tumours in mice
(Trinity College (Dublin, Ireland). Department of Microbiology, 2010)Cancer is a molecular and genetic disease that results from multiple alterations of genes, including proto-oncogenes and tumour-suppressor genes. Virotherapy is a branch of cancer gene therapy where oncolytic viruses are ... -
Development of SFV-based plasmid electroporation for use in tumour treatment and vaccine delivery
(Trinity College (Dublin, Ireland). Department of Microbiology, 2008)Electroporation is the delivery of short and intense electric pulses into living cells (either in tissue or suspension), which results in a transient and reversible increase in permeability of the cell transmembrane. This ... -
Discovery and engineering of antimicrobial peptides to combat food spoilage
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2019)This work sought to use a bioinformatics based approach to discover plant derived antimicrobial peptides which were structurally similar to human β-defensin 3 (HBD3) and which possessed effective antimicrobial activity ... -
Distinct phenotypes and transcriptomes of tup1 and cyc8 mutants suggest novel roles for TUP1 and CYC8 in Saccharomyces cerevisiae
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2023)The Tup1-Cyc8 (Ssn6) co-repressor complex is a regulator of gene transcription in the yeast Saccharomyces cerevisiae. This project provides insights into the distinct roles of Tup1p and Cyc8p in gene repression, identifies ... -
Drug Repurposing as a Strategy to Uncover Effective Antibacterial Compounds using Salmonella as a model
(Trinity College Dublin. School of Genetics & Microbiology. Discipline of Microbiology, 2021)Without doubt, antimicrobial resistance (AMR) is one of the most serious global challenge for public, animals and environmental health. Tackling this huge issue that is threatening modern medicine, is an urgent priority ... -
Effect of intranasal administration of Semliki Forest virus recombinant particles expressing interferon-beta on the progression of experimental autoimmune encephalomyelitis
(Trinity College (Dublin, Ireland). Department of Microbiology, 2006)Multiple sclerosis (MS) is an autoimmune demyelinating disease of the CNS, characterized by the presence of sclerotic lesions throughout the brain. MS is thought to be a CD4+ T helper 1 disease caused by the infiltration ...